Thrombin and IgE antigen induce formation of inositol phosphates by mouse E-mast cells

D. Baranes, Fu-Tong Liu, E. Razin

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Stimulation of murine chondroitin sulfate E containing mast cells (E-MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP1 IP2 and IP3) could be detected 20 s after stimulation. The depletion of Ca2+ from the medium resulted in more than 80% reduction in β-hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP3 under these conditions independent of the presence of extracellular Ca2+.

Original languageEnglish (US)
Pages (from-to)64-68
Number of pages5
JournalFEBS Letters
Volume206
Issue number1
DOIs
StatePublished - Sep 29 1986

Keywords

  • (E-mast cell)
  • Inositol phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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