Abstract
Diets deficient in an indispensable amino acid are known to suppress food intake in rats. Few studies were focused at understanding how amino acid-deficient diets may elicit biochemical changes at the mitochondrial level. The goal of this study was to evaluate mitochondrial function in rats fed diets with 0.00, 0.18, 0.36, and 0.88% threonine (Thr) (set at 0, 30, 60, and 140% of Thr requirement for growth). Here, it is described for the first time that Thr-deficient diets induce a specific uncoupling of mitochondria in liver, especially with NADH-linked substrates, not observed in heart (except for Thr-devoid diet). The advantage of this situation would be to provide ATP to support growth and maintenance when high-quality protein food (or wealth of high-quality food in general) is available, whereas Thr-deficient diets (or deficient-quality protein food) promote the opposite, increasing mitochondrial uncoupling in liver. The uncoupling with NADH substrates would favor the use of nutrients as energy sources with higher FADH-to-NADH ratios, such as fat, minimizing the first irreversible NADH-dependent catabolism of many amino acids, including Thr, thus enhancing the use of the limiting amino acid for protein synthesis when a low quality protein source is available.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 296 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2009 |
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Keywords
- Amino acid deficiency
- Amino acid imbalance
- Mitochondria
- Oxidative phosphorylation
- Oxidative stress
- Oxygen consumption
ASJC Scopus subject areas
- Gastroenterology
- Physiology (medical)
- Physiology
- Hepatology
Cite this
Threonine-deficient diets induced changes in hepatic bioenergetics. / Ross-Inta, Catherine M.; Zhang, Yi Fan; Almendares, Andrew; Giulivi, Cecilia R.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 296, No. 5, 05.2009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Threonine-deficient diets induced changes in hepatic bioenergetics
AU - Ross-Inta, Catherine M.
AU - Zhang, Yi Fan
AU - Almendares, Andrew
AU - Giulivi, Cecilia R
PY - 2009/5
Y1 - 2009/5
N2 - Diets deficient in an indispensable amino acid are known to suppress food intake in rats. Few studies were focused at understanding how amino acid-deficient diets may elicit biochemical changes at the mitochondrial level. The goal of this study was to evaluate mitochondrial function in rats fed diets with 0.00, 0.18, 0.36, and 0.88% threonine (Thr) (set at 0, 30, 60, and 140% of Thr requirement for growth). Here, it is described for the first time that Thr-deficient diets induce a specific uncoupling of mitochondria in liver, especially with NADH-linked substrates, not observed in heart (except for Thr-devoid diet). The advantage of this situation would be to provide ATP to support growth and maintenance when high-quality protein food (or wealth of high-quality food in general) is available, whereas Thr-deficient diets (or deficient-quality protein food) promote the opposite, increasing mitochondrial uncoupling in liver. The uncoupling with NADH substrates would favor the use of nutrients as energy sources with higher FADH-to-NADH ratios, such as fat, minimizing the first irreversible NADH-dependent catabolism of many amino acids, including Thr, thus enhancing the use of the limiting amino acid for protein synthesis when a low quality protein source is available.
AB - Diets deficient in an indispensable amino acid are known to suppress food intake in rats. Few studies were focused at understanding how amino acid-deficient diets may elicit biochemical changes at the mitochondrial level. The goal of this study was to evaluate mitochondrial function in rats fed diets with 0.00, 0.18, 0.36, and 0.88% threonine (Thr) (set at 0, 30, 60, and 140% of Thr requirement for growth). Here, it is described for the first time that Thr-deficient diets induce a specific uncoupling of mitochondria in liver, especially with NADH-linked substrates, not observed in heart (except for Thr-devoid diet). The advantage of this situation would be to provide ATP to support growth and maintenance when high-quality protein food (or wealth of high-quality food in general) is available, whereas Thr-deficient diets (or deficient-quality protein food) promote the opposite, increasing mitochondrial uncoupling in liver. The uncoupling with NADH substrates would favor the use of nutrients as energy sources with higher FADH-to-NADH ratios, such as fat, minimizing the first irreversible NADH-dependent catabolism of many amino acids, including Thr, thus enhancing the use of the limiting amino acid for protein synthesis when a low quality protein source is available.
KW - Amino acid deficiency
KW - Amino acid imbalance
KW - Mitochondria
KW - Oxidative phosphorylation
KW - Oxidative stress
KW - Oxygen consumption
UR - http://www.scopus.com/inward/record.url?scp=66149140594&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66149140594&partnerID=8YFLogxK
U2 - 10.1152/ajpgi.90545.2008
DO - 10.1152/ajpgi.90545.2008
M3 - Article
C2 - 19228885
AN - SCOPUS:66149140594
VL - 296
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 5
ER -