Threonine-deficient diets induced changes in hepatic bioenergetics

Catherine M. Ross-Inta, Yi Fan Zhang, Andrew Almendares, Cecilia R Giulivi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Diets deficient in an indispensable amino acid are known to suppress food intake in rats. Few studies were focused at understanding how amino acid-deficient diets may elicit biochemical changes at the mitochondrial level. The goal of this study was to evaluate mitochondrial function in rats fed diets with 0.00, 0.18, 0.36, and 0.88% threonine (Thr) (set at 0, 30, 60, and 140% of Thr requirement for growth). Here, it is described for the first time that Thr-deficient diets induce a specific uncoupling of mitochondria in liver, especially with NADH-linked substrates, not observed in heart (except for Thr-devoid diet). The advantage of this situation would be to provide ATP to support growth and maintenance when high-quality protein food (or wealth of high-quality food in general) is available, whereas Thr-deficient diets (or deficient-quality protein food) promote the opposite, increasing mitochondrial uncoupling in liver. The uncoupling with NADH substrates would favor the use of nutrients as energy sources with higher FADH-to-NADH ratios, such as fat, minimizing the first irreversible NADH-dependent catabolism of many amino acids, including Thr, thus enhancing the use of the limiting amino acid for protein synthesis when a low quality protein source is available.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume296
Issue number5
DOIs
StatePublished - May 2009

Fingerprint

Threonine
Energy Metabolism
Diet
NAD
Food Quality
Liver
Amino Acids
Proteins
Liver Mitochondrion
Growth
Eating
Adenosine Triphosphate
Fats
Maintenance
Food

Keywords

  • Amino acid deficiency
  • Amino acid imbalance
  • Mitochondria
  • Oxidative phosphorylation
  • Oxidative stress
  • Oxygen consumption

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology (medical)
  • Physiology
  • Hepatology

Cite this

Threonine-deficient diets induced changes in hepatic bioenergetics. / Ross-Inta, Catherine M.; Zhang, Yi Fan; Almendares, Andrew; Giulivi, Cecilia R.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 296, No. 5, 05.2009.

Research output: Contribution to journalArticle

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