Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone

Michael J. Evans, Laura S. Van Winkle, Michelle V. Fanucchi, Elina Toskala, Emily C. Luck, Philip L. Sannes, Charles Plopper

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The airway basement membrane zone is a region specialized for the attachment of the epithelium with the matrix. The epithelium is attached to the lamina densa, which, in turn, is connected to types I and III collagen of the lamina reticularis with anchoring fibrils. The purpose of this study was to define the three-dimensional organization of the structural proteins of the lamina reticularis in the rat trachea. We approached this problem by using whole mounts to look down on the flat surface of the basement-membrane zone rather than a cross section of its thin profile. Fluorescent microscopy with long working distance water immersion objectives and scanning electron microscopy revealed that the structural proteins are arranged as a mat of large fibers oriented along the longitudinal axis of the airway. Smaller fibers are crosslinked with the larger fibers to complete this structure. Other small fibers are oriented around the large fibers and an amorphous material covers individual fibers. The large fibers oriented along the longitudinal axis of the airway are consistent with prior descriptions of fibers composed of collagen III with some collagen I and V; small fibers encircling the large fibers may be collagen VI. The crosslinking fibers are made up of elastin and probably elastin-associated microfibrils. The amorphous proteins covering the fibrous framework may contain proteoglycans and other nonstructural proteins reported to be in the lamina reticularis. The present studies demonstrate that the structural proteins of the lamina reticularis in the rat trachea are arranged as fibers in a highly organized manner.

Original languageEnglish (US)
Pages (from-to)393-397
Number of pages5
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume22
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Basement Membrane
Rats
Collagen
Elastin
Fibers
Trachea
Proteins
Epithelium
Scleroproteins
Microfibrils
Collagen Type III
Immersion
Proteoglycans
Collagen Type I
Electron Scanning Microscopy
Microscopy
Water
Crosslinking
Microscopic examination
Scanning electron microscopy

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Evans, M. J., Van Winkle, L. S., Fanucchi, M. V., Toskala, E., Luck, E. C., Sannes, P. L., & Plopper, C. (2000). Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone. American Journal of Respiratory Cell and Molecular Biology, 22(4), 393-397.

Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone. / Evans, Michael J.; Van Winkle, Laura S.; Fanucchi, Michelle V.; Toskala, Elina; Luck, Emily C.; Sannes, Philip L.; Plopper, Charles.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 22, No. 4, 2000, p. 393-397.

Research output: Contribution to journalArticle

Evans, MJ, Van Winkle, LS, Fanucchi, MV, Toskala, E, Luck, EC, Sannes, PL & Plopper, C 2000, 'Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone', American Journal of Respiratory Cell and Molecular Biology, vol. 22, no. 4, pp. 393-397.
Evans, Michael J. ; Van Winkle, Laura S. ; Fanucchi, Michelle V. ; Toskala, Elina ; Luck, Emily C. ; Sannes, Philip L. ; Plopper, Charles. / Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone. In: American Journal of Respiratory Cell and Molecular Biology. 2000 ; Vol. 22, No. 4. pp. 393-397.
@article{df4636f3144a46d5bddd75c24f732044,
title = "Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone",
abstract = "The airway basement membrane zone is a region specialized for the attachment of the epithelium with the matrix. The epithelium is attached to the lamina densa, which, in turn, is connected to types I and III collagen of the lamina reticularis with anchoring fibrils. The purpose of this study was to define the three-dimensional organization of the structural proteins of the lamina reticularis in the rat trachea. We approached this problem by using whole mounts to look down on the flat surface of the basement-membrane zone rather than a cross section of its thin profile. Fluorescent microscopy with long working distance water immersion objectives and scanning electron microscopy revealed that the structural proteins are arranged as a mat of large fibers oriented along the longitudinal axis of the airway. Smaller fibers are crosslinked with the larger fibers to complete this structure. Other small fibers are oriented around the large fibers and an amorphous material covers individual fibers. The large fibers oriented along the longitudinal axis of the airway are consistent with prior descriptions of fibers composed of collagen III with some collagen I and V; small fibers encircling the large fibers may be collagen VI. The crosslinking fibers are made up of elastin and probably elastin-associated microfibrils. The amorphous proteins covering the fibrous framework may contain proteoglycans and other nonstructural proteins reported to be in the lamina reticularis. The present studies demonstrate that the structural proteins of the lamina reticularis in the rat trachea are arranged as fibers in a highly organized manner.",
author = "Evans, {Michael J.} and {Van Winkle}, {Laura S.} and Fanucchi, {Michelle V.} and Elina Toskala and Luck, {Emily C.} and Sannes, {Philip L.} and Charles Plopper",
year = "2000",
language = "English (US)",
volume = "22",
pages = "393--397",
journal = "American Journal of Respiratory Cell and Molecular Biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "4",

}

TY - JOUR

T1 - Three-dimensional organization of the lamina reticularis in the rat tracheal basement membrane zone

AU - Evans, Michael J.

AU - Van Winkle, Laura S.

AU - Fanucchi, Michelle V.

AU - Toskala, Elina

AU - Luck, Emily C.

AU - Sannes, Philip L.

AU - Plopper, Charles

PY - 2000

Y1 - 2000

N2 - The airway basement membrane zone is a region specialized for the attachment of the epithelium with the matrix. The epithelium is attached to the lamina densa, which, in turn, is connected to types I and III collagen of the lamina reticularis with anchoring fibrils. The purpose of this study was to define the three-dimensional organization of the structural proteins of the lamina reticularis in the rat trachea. We approached this problem by using whole mounts to look down on the flat surface of the basement-membrane zone rather than a cross section of its thin profile. Fluorescent microscopy with long working distance water immersion objectives and scanning electron microscopy revealed that the structural proteins are arranged as a mat of large fibers oriented along the longitudinal axis of the airway. Smaller fibers are crosslinked with the larger fibers to complete this structure. Other small fibers are oriented around the large fibers and an amorphous material covers individual fibers. The large fibers oriented along the longitudinal axis of the airway are consistent with prior descriptions of fibers composed of collagen III with some collagen I and V; small fibers encircling the large fibers may be collagen VI. The crosslinking fibers are made up of elastin and probably elastin-associated microfibrils. The amorphous proteins covering the fibrous framework may contain proteoglycans and other nonstructural proteins reported to be in the lamina reticularis. The present studies demonstrate that the structural proteins of the lamina reticularis in the rat trachea are arranged as fibers in a highly organized manner.

AB - The airway basement membrane zone is a region specialized for the attachment of the epithelium with the matrix. The epithelium is attached to the lamina densa, which, in turn, is connected to types I and III collagen of the lamina reticularis with anchoring fibrils. The purpose of this study was to define the three-dimensional organization of the structural proteins of the lamina reticularis in the rat trachea. We approached this problem by using whole mounts to look down on the flat surface of the basement-membrane zone rather than a cross section of its thin profile. Fluorescent microscopy with long working distance water immersion objectives and scanning electron microscopy revealed that the structural proteins are arranged as a mat of large fibers oriented along the longitudinal axis of the airway. Smaller fibers are crosslinked with the larger fibers to complete this structure. Other small fibers are oriented around the large fibers and an amorphous material covers individual fibers. The large fibers oriented along the longitudinal axis of the airway are consistent with prior descriptions of fibers composed of collagen III with some collagen I and V; small fibers encircling the large fibers may be collagen VI. The crosslinking fibers are made up of elastin and probably elastin-associated microfibrils. The amorphous proteins covering the fibrous framework may contain proteoglycans and other nonstructural proteins reported to be in the lamina reticularis. The present studies demonstrate that the structural proteins of the lamina reticularis in the rat trachea are arranged as fibers in a highly organized manner.

UR - http://www.scopus.com/inward/record.url?scp=0034036585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034036585&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 393

EP - 397

JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

IS - 4

ER -