Three-dimensional mapping of smooth muscle in the distal conducting airways of mouse, rabbit, and monkey

S. M. Smiley-Jewell, M. U. Tran, A. J. Weir, Z. A. Johnson, L. S. Van Winkle, Charles Plopper

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Airway smooth muscle remodeling is implicated in a number of constrictive pulmonary diseases such as asthma and may include changes in smooth muscle orientation and abundance. Both factors were compared in the normal distal bronchioles of the mouse, rabbit, and rhesus monkey (respiratory bronchioles included). Airway smooth muscle was measured by using a three-dimensional approach employing confocal microscopy and whole-mount cytochemistry with fluorochrome-conjugated phalloidin, a probe for polymerized actin. Smooth muscle orientation had a wide range of angles along the airway, but the distribution was conserved among species and among distal airway generations. At the bifurcation of proximal bronchioles, smooth muscle was nearly parallel to the longitudinal axis of the airway. Smooth muscle abundance was significantly different between species (abundance was less in the monkey compared with the mouse and rabbit), and there was a trend for abundance to decrease with each more distal airway generation. This study defines the normal distribution of smooth muscle in three test species and provides a basis for future comparisons with the diseased state.

Original languageEnglish (US)
Pages (from-to)1506-1514
Number of pages9
JournalJournal of Applied Physiology
Volume93
Issue number4
StatePublished - Oct 2002

Fingerprint

Haplorhini
Smooth Muscle
Rabbits
Bronchioles
Phalloidine
Histocytochemistry
Normal Distribution
Macaca mulatta
Fluorescent Dyes
Confocal Microscopy
Lung Diseases
Actins
Asthma

Keywords

  • Actin
  • Bronchiole
  • Lung
  • Peripheral airways
  • Species comparison

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Smiley-Jewell, S. M., Tran, M. U., Weir, A. J., Johnson, Z. A., Van Winkle, L. S., & Plopper, C. (2002). Three-dimensional mapping of smooth muscle in the distal conducting airways of mouse, rabbit, and monkey. Journal of Applied Physiology, 93(4), 1506-1514.

Three-dimensional mapping of smooth muscle in the distal conducting airways of mouse, rabbit, and monkey. / Smiley-Jewell, S. M.; Tran, M. U.; Weir, A. J.; Johnson, Z. A.; Van Winkle, L. S.; Plopper, Charles.

In: Journal of Applied Physiology, Vol. 93, No. 4, 10.2002, p. 1506-1514.

Research output: Contribution to journalArticle

Smiley-Jewell, SM, Tran, MU, Weir, AJ, Johnson, ZA, Van Winkle, LS & Plopper, C 2002, 'Three-dimensional mapping of smooth muscle in the distal conducting airways of mouse, rabbit, and monkey', Journal of Applied Physiology, vol. 93, no. 4, pp. 1506-1514.
Smiley-Jewell, S. M. ; Tran, M. U. ; Weir, A. J. ; Johnson, Z. A. ; Van Winkle, L. S. ; Plopper, Charles. / Three-dimensional mapping of smooth muscle in the distal conducting airways of mouse, rabbit, and monkey. In: Journal of Applied Physiology. 2002 ; Vol. 93, No. 4. pp. 1506-1514.
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