Abstract
Growth patterns of a number of human tumor cell lines that form three- dimensional structures of various architectures when cultured without carrier heads in a NASA rotary cell culture system are described and illustrated. The culture system, which was designed to mimic microgravity, maintained cells in suspension under very low-shear stress throughout culture. Spheroid (particulate) production occurred within a few hours after culture was started, and spheroids increased in size by cell division and fusion of small spheroids, usually stabilizing at a spheroid diameter of about 0.5 ram. Architecture of spheroids varied with cell type. Cellular interactions that occurred in spheroids resulted in conformation and shape changes of cells, and some cell lines produced complex, epithelial-like architectures. Expression of the cell adhesion molecules, CD44 and E cadherin, was upregulated in the three-dimensional constructs. Coculture of fibroblast spheroids with PC3 prostate cancer cells induced tenasein expression by the fibroblasts underlying tire adherent prostate epithelial cells. Invasion of the fibroblast spheroids by the malignant epithelium was also demonstrated.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 459-466 |
| Number of pages | 8 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 33 |
| Issue number | 6 |
| State | Published - Jun 1997 |
| Externally published | Yes |
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Keywords
- Low-shear culture
- Spheroid architecture
- Suspension culture
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology
Cite this
Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor. / Ingram, M.; Techy, G. B.; Saroufeem, R.; Yazan, O.; Narayan, K. S.; Goodwin, T. J.; Spaulding, G. F.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 33, No. 6, 06.1997, p. 459-466.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor
AU - Ingram, M.
AU - Techy, G. B.
AU - Saroufeem, R.
AU - Yazan, O.
AU - Narayan, K. S.
AU - Goodwin, T. J.
AU - Spaulding, G. F.
PY - 1997/6
Y1 - 1997/6
N2 - Growth patterns of a number of human tumor cell lines that form three- dimensional structures of various architectures when cultured without carrier heads in a NASA rotary cell culture system are described and illustrated. The culture system, which was designed to mimic microgravity, maintained cells in suspension under very low-shear stress throughout culture. Spheroid (particulate) production occurred within a few hours after culture was started, and spheroids increased in size by cell division and fusion of small spheroids, usually stabilizing at a spheroid diameter of about 0.5 ram. Architecture of spheroids varied with cell type. Cellular interactions that occurred in spheroids resulted in conformation and shape changes of cells, and some cell lines produced complex, epithelial-like architectures. Expression of the cell adhesion molecules, CD44 and E cadherin, was upregulated in the three-dimensional constructs. Coculture of fibroblast spheroids with PC3 prostate cancer cells induced tenasein expression by the fibroblasts underlying tire adherent prostate epithelial cells. Invasion of the fibroblast spheroids by the malignant epithelium was also demonstrated.
AB - Growth patterns of a number of human tumor cell lines that form three- dimensional structures of various architectures when cultured without carrier heads in a NASA rotary cell culture system are described and illustrated. The culture system, which was designed to mimic microgravity, maintained cells in suspension under very low-shear stress throughout culture. Spheroid (particulate) production occurred within a few hours after culture was started, and spheroids increased in size by cell division and fusion of small spheroids, usually stabilizing at a spheroid diameter of about 0.5 ram. Architecture of spheroids varied with cell type. Cellular interactions that occurred in spheroids resulted in conformation and shape changes of cells, and some cell lines produced complex, epithelial-like architectures. Expression of the cell adhesion molecules, CD44 and E cadherin, was upregulated in the three-dimensional constructs. Coculture of fibroblast spheroids with PC3 prostate cancer cells induced tenasein expression by the fibroblasts underlying tire adherent prostate epithelial cells. Invasion of the fibroblast spheroids by the malignant epithelium was also demonstrated.
KW - Low-shear culture
KW - Spheroid architecture
KW - Suspension culture
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M3 - Article
C2 - 9201514
AN - SCOPUS:0030970721
VL - 33
SP - 459
EP - 466
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 6
ER -