Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor

M. Ingram, G. B. Techy, R. Saroufeem, O. Yazan, K. S. Narayan, T. J. Goodwin, G. F. Spaulding

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Growth patterns of a number of human tumor cell lines that form three- dimensional structures of various architectures when cultured without carrier heads in a NASA rotary cell culture system are described and illustrated. The culture system, which was designed to mimic microgravity, maintained cells in suspension under very low-shear stress throughout culture. Spheroid (particulate) production occurred within a few hours after culture was started, and spheroids increased in size by cell division and fusion of small spheroids, usually stabilizing at a spheroid diameter of about 0.5 ram. Architecture of spheroids varied with cell type. Cellular interactions that occurred in spheroids resulted in conformation and shape changes of cells, and some cell lines produced complex, epithelial-like architectures. Expression of the cell adhesion molecules, CD44 and E cadherin, was upregulated in the three-dimensional constructs. Coculture of fibroblast spheroids with PC3 prostate cancer cells induced tenasein expression by the fibroblasts underlying tire adherent prostate epithelial cells. Invasion of the fibroblast spheroids by the malignant epithelium was also demonstrated.

Original languageEnglish (US)
Pages (from-to)459-466
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume33
Issue number6
StatePublished - Jun 1997
Externally publishedYes

Fingerprint

Weightlessness
Microgravity
Bioreactors
Tumor Cell Line
Tumors
Fibroblasts
Cells
Growth
Cell culture
United States National Aeronautics and Space Administration
Cell Shape
Cell Fusion
Cell Adhesion Molecules
Cadherins
Coculture Techniques
Cell Division
Prostate
Prostatic Neoplasms
Suspensions
Epithelium

Keywords

  • Low-shear culture
  • Spheroid architecture
  • Suspension culture

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Ingram, M., Techy, G. B., Saroufeem, R., Yazan, O., Narayan, K. S., Goodwin, T. J., & Spaulding, G. F. (1997). Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor. In Vitro Cellular and Developmental Biology - Animal, 33(6), 459-466.

Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor. / Ingram, M.; Techy, G. B.; Saroufeem, R.; Yazan, O.; Narayan, K. S.; Goodwin, T. J.; Spaulding, G. F.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 33, No. 6, 06.1997, p. 459-466.

Research output: Contribution to journalArticle

Ingram, M, Techy, GB, Saroufeem, R, Yazan, O, Narayan, KS, Goodwin, TJ & Spaulding, GF 1997, 'Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor', In Vitro Cellular and Developmental Biology - Animal, vol. 33, no. 6, pp. 459-466.
Ingram, M. ; Techy, G. B. ; Saroufeem, R. ; Yazan, O. ; Narayan, K. S. ; Goodwin, T. J. ; Spaulding, G. F. / Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a nasa bioreactor. In: In Vitro Cellular and Developmental Biology - Animal. 1997 ; Vol. 33, No. 6. pp. 459-466.
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