Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation

Y. S. Hwang, E. E. Karrer, B. R. Thomas, L. Chen, R. L. Rodriguez

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Expression of α-amylase genes during seedling development plays a key role in production of sugar from the starch stored in the cereal seed. Rice α-amylase Amy3D promoter/GUS constructs in transgenic rice cell lines were studied to identity cis elements in the promoter of this metabolite-regulated gene. Three sequences having the greatest effects on Amy3D gene expression included the amylase element (TATCCAT), the CGACG element, and a G box-related element (CTACGTGGCCA). These promoter cis elements are needed for high-level expression of Amy3D under conditions of sugar starvation. The involvement of G box cis-elements in environmental stress responses suggest a link between the nutrient stress and the environmental stress responses of the plant.

Original languageEnglish (US)
Pages (from-to)331-341
Number of pages11
JournalPlant Molecular Biology
Volume36
Issue number3
DOIs
StatePublished - Feb 1998

Keywords

  • Cereal seedling
  • Environmental stress
  • G box
  • Signal metabolite hypothesis
  • Source/sink
  • Transgenic cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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    Hwang, Y. S., Karrer, E. E., Thomas, B. R., Chen, L., & Rodriguez, R. L. (1998). Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation. Plant Molecular Biology, 36(3), 331-341. https://doi.org/10.1023/A:1005956104636