Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation

Y. S. Hwang, E. E. Karrer, B. R. Thomas, L. Chen, R. L. Rodriguez

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Expression of α-amylase genes during seedling development plays a key role in production of sugar from the starch stored in the cereal seed. Rice α-amylase Amy3D promoter/GUS constructs in transgenic rice cell lines were studied to identity cis elements in the promoter of this metabolite-regulated gene. Three sequences having the greatest effects on Amy3D gene expression included the amylase element (TATCCAT), the CGACG element, and a G box-related element (CTACGTGGCCA). These promoter cis elements are needed for high-level expression of Amy3D under conditions of sugar starvation. The involvement of G box cis-elements in environmental stress responses suggest a link between the nutrient stress and the environmental stress responses of the plant.

Original languageEnglish (US)
Pages (from-to)331-341
Number of pages11
JournalPlant Molecular Biology
Volume36
Issue number3
DOIs
StatePublished - Feb 1998

Fingerprint

Amylases
Starvation
amylases
Sugars
starvation
promoter regions
sugars
rice
stress response
Genes
Gene Expression
Metabolites
Seedlings
Gene expression
Starch
Nutrients
Seed
Seeds
genes
Cells

Keywords

  • Cereal seedling
  • Environmental stress
  • G box
  • Signal metabolite hypothesis
  • Source/sink
  • Transgenic cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Hwang, Y. S., Karrer, E. E., Thomas, B. R., Chen, L., & Rodriguez, R. L. (1998). Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation. Plant Molecular Biology, 36(3), 331-341. https://doi.org/10.1023/A:1005956104636

Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation. / Hwang, Y. S.; Karrer, E. E.; Thomas, B. R.; Chen, L.; Rodriguez, R. L.

In: Plant Molecular Biology, Vol. 36, No. 3, 02.1998, p. 331-341.

Research output: Contribution to journalArticle

Hwang, YS, Karrer, EE, Thomas, BR, Chen, L & Rodriguez, RL 1998, 'Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation', Plant Molecular Biology, vol. 36, no. 3, pp. 331-341. https://doi.org/10.1023/A:1005956104636
Hwang, Y. S. ; Karrer, E. E. ; Thomas, B. R. ; Chen, L. ; Rodriguez, R. L. / Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation. In: Plant Molecular Biology. 1998 ; Vol. 36, No. 3. pp. 331-341.
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