Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Umit Hakan Yildiz, Hans Peter M De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani, Bo Liedberg

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

Original languageEnglish (US)
Pages (from-to)442-447
Number of pages6
JournalSmall
Volume10
Issue number3
DOIs
StatePublished - Feb 12 2014

Fingerprint

Phosphatases
Enzyme Assays
Labels
Assays
Enzymes
Adenosine Triphosphate
Alkaline Phosphatase
Polymers
Thiophene
Amides
Block copolymers
Thiophenes
Quenching
Hydrolysis
Polystyrenes
Fluorescence
Membranes
Recovery
Monitoring

Keywords

  • ATP sensor
  • enzymatic reactions
  • polymersomes
  • vesicular compartments
  • water-soluble polythiophene

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Yildiz, U. H., De Hoog, H. P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., & Liedberg, B. (2014). Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments. Small, 10(3), 442-447. https://doi.org/10.1002/smll.201300060

Third-party ATP sensing in polymersomes : A label-free assay of enzyme reactions in vesicular compartments. / Yildiz, Umit Hakan; De Hoog, Hans Peter M; Fu, Zhikang; Tomczak, Nikodem; Parikh, Atul N.; Nallani, Madhavan; Liedberg, Bo.

In: Small, Vol. 10, No. 3, 12.02.2014, p. 442-447.

Research output: Contribution to journalArticle

Yildiz, UH, De Hoog, HPM, Fu, Z, Tomczak, N, Parikh, AN, Nallani, M & Liedberg, B 2014, 'Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments', Small, vol. 10, no. 3, pp. 442-447. https://doi.org/10.1002/smll.201300060
Yildiz UH, De Hoog HPM, Fu Z, Tomczak N, Parikh AN, Nallani M et al. Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments. Small. 2014 Feb 12;10(3):442-447. https://doi.org/10.1002/smll.201300060
Yildiz, Umit Hakan ; De Hoog, Hans Peter M ; Fu, Zhikang ; Tomczak, Nikodem ; Parikh, Atul N. ; Nallani, Madhavan ; Liedberg, Bo. / Third-party ATP sensing in polymersomes : A label-free assay of enzyme reactions in vesicular compartments. In: Small. 2014 ; Vol. 10, No. 3. pp. 442-447.
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