Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Umit Hakan Yildiz; Hans Peter M De Hoog; Zhikang Fu; Nikodem Tomczak; Atul N. Parikh; Madhavan Nallani; Bo Liedberg

doi:10.1002/smll.201300060

Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Umit Hakan Yildiz, Hans Peter M De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani, Bo Liedberg

Animal Science

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

Original language	English (US)
Pages (from-to)	442-447
Number of pages	6
Journal	Small
Volume	10
Issue number	3
DOIs	https://doi.org/10.1002/smll.201300060
State	Published - Feb 12 2014

Keywords

ATP sensor
enzymatic reactions
polymersomes
vesicular compartments
water-soluble polythiophene

ASJC Scopus subject areas

Biomaterials
Engineering (miscellaneous)
Biotechnology
Medicine(all)

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Yildiz, U. H., De Hoog, H. P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., & Liedberg, B. (2014). Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments. Small, 10(3), 442-447. https://doi.org/10.1002/smll.201300060

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AU - Parikh, Atul N.

AU - Nallani, Madhavan

AU - Liedberg, Bo

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