Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Umit Hakan Yildiz, Hans Peter M De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani, Bo Liedberg

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

Original languageEnglish (US)
Pages (from-to)442-447
Number of pages6
JournalSmall
Volume10
Issue number3
DOIs
StatePublished - Feb 12 2014

Keywords

  • ATP sensor
  • enzymatic reactions
  • polymersomes
  • vesicular compartments
  • water-soluble polythiophene

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

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  • Cite this

    Yildiz, U. H., De Hoog, H. P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., & Liedberg, B. (2014). Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments. Small, 10(3), 442-447. https://doi.org/10.1002/smll.201300060