Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Umit Hakan Yildiz; Hans Peter M De Hoog; Zhikang Fu; Nikodem Tomczak; Atul N. Parikh; Madhavan Nallani; Bo Liedberg

doi:10.1002/smll.201300060

Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Umit Hakan Yildiz, Hans Peter M De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani, Bo Liedberg

Animal Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

Original language	English (US)
Pages (from-to)	442-447
Number of pages	6
Journal	Small
Volume	10
Issue number	3
DOIs	https://doi.org/10.1002/smll.201300060
State	Published - Feb 12 2014

Keywords

ATP sensor
enzymatic reactions
polymersomes
vesicular compartments
water-soluble polythiophene

ASJC Scopus subject areas

Biomaterials
Engineering (miscellaneous)
Biotechnology
Medicine(all)

Access to Document

10.1002/smll.201300060

Fingerprint Dive into the research topics of 'Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments'. Together they form a unique fingerprint.

Cite this

@article{bb10246dec4e4c57bbbae79e19f0db0d,

title = "Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments",

abstract = "Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.",

keywords = "ATP sensor, enzymatic reactions, polymersomes, vesicular compartments, water-soluble polythiophene",

author = "Yildiz, {Umit Hakan} and {De Hoog}, {Hans Peter M} and Zhikang Fu and Nikodem Tomczak and Parikh, {Atul N.} and Madhavan Nallani and Bo Liedberg",

year = "2014",

month = feb,

day = "12",

doi = "10.1002/smll.201300060",

language = "English (US)",

volume = "10",

pages = "442--447",

journal = "Small",

issn = "1613-6810",

publisher = "Wiley-VCH Verlag",

number = "3",

}

TY - JOUR

T1 - Third-party ATP sensing in polymersomes

T2 - A label-free assay of enzyme reactions in vesicular compartments

AU - Yildiz, Umit Hakan

AU - De Hoog, Hans Peter M

AU - Fu, Zhikang

AU - Tomczak, Nikodem

AU - Parikh, Atul N.

AU - Nallani, Madhavan

AU - Liedberg, Bo

PY - 2014/2/12

Y1 - 2014/2/12

N2 - Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

AB - Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

KW - ATP sensor

KW - enzymatic reactions

KW - polymersomes

KW - vesicular compartments

KW - water-soluble polythiophene

UR - http://www.scopus.com/inward/record.url?scp=84893454196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893454196&partnerID=8YFLogxK

U2 - 10.1002/smll.201300060

DO - 10.1002/smll.201300060

M3 - Article

C2 - 23963775

AN - SCOPUS:84893454196

VL - 10

SP - 442

EP - 447

JO - Small

JF - Small

SN - 1613-6810

IS - 3

ER -

Third-party ATP sensing in polymersomes: A label-free assay of enzyme reactions in vesicular compartments

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this