Abstract
Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.
Original language | English (US) |
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Pages (from-to) | 442-447 |
Number of pages | 6 |
Journal | Small |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - Feb 12 2014 |
Keywords
- ATP sensor
- enzymatic reactions
- polymersomes
- vesicular compartments
- water-soluble polythiophene
ASJC Scopus subject areas
- Biomaterials
- Engineering (miscellaneous)
- Biotechnology
- Medicine(all)