Abstract
Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 442-447 |
| Number of pages | 6 |
| Journal | Small |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 12 2014 |
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Keywords
- ATP sensor
- enzymatic reactions
- polymersomes
- vesicular compartments
- water-soluble polythiophene
ASJC Scopus subject areas
- Biomaterials
- Engineering (miscellaneous)
- Biotechnology
- Medicine(all)
Cite this
Third-party ATP sensing in polymersomes : A label-free assay of enzyme reactions in vesicular compartments. / Yildiz, Umit Hakan; De Hoog, Hans Peter M; Fu, Zhikang; Tomczak, Nikodem; Parikh, Atul N.; Nallani, Madhavan; Liedberg, Bo.
In: Small, Vol. 10, No. 3, 12.02.2014, p. 442-447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Third-party ATP sensing in polymersomes
T2 - A label-free assay of enzyme reactions in vesicular compartments
AU - Yildiz, Umit Hakan
AU - De Hoog, Hans Peter M
AU - Fu, Zhikang
AU - Tomczak, Nikodem
AU - Parikh, Atul N.
AU - Nallani, Madhavan
AU - Liedberg, Bo
PY - 2014/2/12
Y1 - 2014/2/12
N2 - Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.
AB - Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.
KW - ATP sensor
KW - enzymatic reactions
KW - polymersomes
KW - vesicular compartments
KW - water-soluble polythiophene
UR - http://www.scopus.com/inward/record.url?scp=84893454196&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893454196&partnerID=8YFLogxK
U2 - 10.1002/smll.201300060
DO - 10.1002/smll.201300060
M3 - Article
C2 - 23963775
AN - SCOPUS:84893454196
VL - 10
SP - 442
EP - 447
JO - Small
JF - Small
SN - 1613-6810
IS - 3
ER -