Therapeutic protective effects of IL-12 combined with soluble IL-4 receptor against established infections of herpes simplex virus type 1 in thermally injured mice

Hiroyuki Kobayashi, Makiko Kobayashi, Tokuichiro Utsunomiya, David N. Herndon, Richard B Pollard, Fujio Suzuki

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Abstract

The effect of combination therapy between IL-12 and soluble IL-4R (sIL- 4R) on the established infection of HSV-1 in thermally injured mice (TI mice) was investigated. All of the TI mice infected with lethal amounts of HSV-1 died when IL-12 was given therapeutically at a dose of 500 U/mouse. However, 80% of these mice treated prophylactically with IL-12 survived compared with 0% survival of the same mice treated with saline. The therapeutic administration of IL-12 to TI mice currently infected with HSV-1 caused an 80% survival of these mice when the treatment was combined with sIL-4R. Although IL-12 did not stimulate IFN-γ production in cultures of splenic T cells from TI mice, IFN-γ was produced by stimulation with IL-12 when the producer cells were prepared from TI mice that had been treated previously with sIL-4R. After stimulation with anti-CD3 mAb, splenic T cells from TI mice with the established infection of HSV-1 produced IL-4 into their culture fluids. However, IL-4 was not produced by splenic T cells that were prepared from the same infected mice treated with IL-12 and sIL-4R in combination. The results obtained herein indicate that the efficacies of the combination therapy against the established infection of HSV-1 may result from the IFN- γ production stimulated by IL-12 in TI mice that are treated with sIL-4R for reducing burn-associated type 2 T cell responses.

Original languageEnglish (US)
Pages (from-to)7148-7154
Number of pages7
JournalJournal of Immunology
Volume162
Issue number12
StatePublished - Jun 15 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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