Therapeutic effect of RhTNFR: Fc on rats with adjuvant arthritis and its effect on peritoneal macrophages

Yu Jing Wu, Yun Fang Zhang, Ling Ling Zhang, Qingtong Wang, Shan Shan Song, Bei Huang, Wei Wei

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the effects of RhTNFR: Fc in an established adjuvant arthritis (AA) rat model and the effects on cytokines production of peritoneal macrophage (PMφ). Methods: AA was induced by complete Freund adjuvant. AA rats were randomly divided into different groups and then treated with RhTNFR: Fc (1, 3, 9 mg·kg-1), or IgG-Fc (9 mg·kg-1), respectively after immunization. Arthritis was evaluated by global assessment, polyarthritis index, hind paw swelling, swollen joint count, X-ray of the paws and histopathological examination and histopathological scores. Activities of interleukin-1β (IL-1β) and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in supernate of PMφ were assessed by ELISA. Results: Administration of RhTNFR: Fc significantly reduced the global assessment score, polyarthritis index, hind paw swelling and swollen joint count, ameliorated X-ray and histopathological manifestations, and decreased the expressions of IL-1β, IL-6 and TNF-α. Conclusion: Data presented here demonstrate that administration of RhTNFR: Fc significantly attenuates progression of experimental arthritis, and reduces proinflammatory cytokines of PMφ.

Original languageEnglish (US)
Pages (from-to)524-530
Number of pages7
JournalChinese Journal of New Drugs
Volume22
Issue number5
StatePublished - Mar 15 2013
Externally publishedYes

Keywords

  • Adjuvant arthritis
  • Cytokines
  • Inflammation
  • Macrophage
  • Pathology
  • TNF-α

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Wu, Y. J., Zhang, Y. F., Zhang, L. L., Wang, Q., Song, S. S., Huang, B., & Wei, W. (2013). Therapeutic effect of RhTNFR: Fc on rats with adjuvant arthritis and its effect on peritoneal macrophages. Chinese Journal of New Drugs, 22(5), 524-530.