Abstract
Cerenkov luminescence imaging (CLI) is an optical technique for imaging radiolabeled molecules in vivo. It has demonstrated utility in both the clinical and preclinical settings and can serve as a substitute for nuclear imaging instrumentation in some cases. However, optical scattering fundamentally limits the resolution and depth of imaging that can be achieved with this modality. In this Letter, we report the numerical results that support the potential for ultrasound-modulated Cerenkov luminescence imaging (USCLI), a new imaging modality that can mitigate optical scattering. The technique uses an acoustic field to modulate the refractive index of the medium and, thus, the intensity of Cerenkov luminescence in a spatially precise manner. This mechanism of contrast has not been reported previously. For a physiologically compatible ultrasound peak pressure of 1 MPa, ∼0.1% of the Cerenkov signal can be modulated. Furthermore, our simulations show that USCLI can overcome the scattering limit of resolution for CLI and provide higher-resolution imaging. For an18F point source centered in a 1 cm3 simulated tissue phantom with a scattering coefficient of μs 0 10 cm−1, <2 mm full width at half-maximum lateral spatial resolution is possible, a resolution three times finer than the same phantom imaged with CLI.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3509-3512 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 43 |
| Issue number | 15 |
| DOIs | |
| State | Published - Aug 1 2018 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Theoretical investigation of ultrasound-modulated Cerenkov luminescence imaging for higher-resolution imaging in turbid media. / Klein, Justin S.; Mitchell, Gregory; Stephens, Douglas N.; Cherry, Simon R.
In: Optics Letters, Vol. 43, No. 15, 01.08.2018, p. 3509-3512.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Theoretical investigation of ultrasound-modulated Cerenkov luminescence imaging for higher-resolution imaging in turbid media
AU - Klein, Justin S.
AU - Mitchell, Gregory
AU - Stephens, Douglas N.
AU - Cherry, Simon R
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Cerenkov luminescence imaging (CLI) is an optical technique for imaging radiolabeled molecules in vivo. It has demonstrated utility in both the clinical and preclinical settings and can serve as a substitute for nuclear imaging instrumentation in some cases. However, optical scattering fundamentally limits the resolution and depth of imaging that can be achieved with this modality. In this Letter, we report the numerical results that support the potential for ultrasound-modulated Cerenkov luminescence imaging (USCLI), a new imaging modality that can mitigate optical scattering. The technique uses an acoustic field to modulate the refractive index of the medium and, thus, the intensity of Cerenkov luminescence in a spatially precise manner. This mechanism of contrast has not been reported previously. For a physiologically compatible ultrasound peak pressure of 1 MPa, ∼0.1% of the Cerenkov signal can be modulated. Furthermore, our simulations show that USCLI can overcome the scattering limit of resolution for CLI and provide higher-resolution imaging. For an18F point source centered in a 1 cm3 simulated tissue phantom with a scattering coefficient of μs 0 10 cm−1, <2 mm full width at half-maximum lateral spatial resolution is possible, a resolution three times finer than the same phantom imaged with CLI.
AB - Cerenkov luminescence imaging (CLI) is an optical technique for imaging radiolabeled molecules in vivo. It has demonstrated utility in both the clinical and preclinical settings and can serve as a substitute for nuclear imaging instrumentation in some cases. However, optical scattering fundamentally limits the resolution and depth of imaging that can be achieved with this modality. In this Letter, we report the numerical results that support the potential for ultrasound-modulated Cerenkov luminescence imaging (USCLI), a new imaging modality that can mitigate optical scattering. The technique uses an acoustic field to modulate the refractive index of the medium and, thus, the intensity of Cerenkov luminescence in a spatially precise manner. This mechanism of contrast has not been reported previously. For a physiologically compatible ultrasound peak pressure of 1 MPa, ∼0.1% of the Cerenkov signal can be modulated. Furthermore, our simulations show that USCLI can overcome the scattering limit of resolution for CLI and provide higher-resolution imaging. For an18F point source centered in a 1 cm3 simulated tissue phantom with a scattering coefficient of μs 0 10 cm−1, <2 mm full width at half-maximum lateral spatial resolution is possible, a resolution three times finer than the same phantom imaged with CLI.
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U2 - 10.1364/OL.43.003509
DO - 10.1364/OL.43.003509
M3 - Article
C2 - 30067696
AN - SCOPUS:85050922886
VL - 43
SP - 3509
EP - 3512
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 15
ER -