Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli: Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission

Steve L. Stockham, A. M. Kjemtrup, Patricia A Conrad, D. A. Schmidt, M. A. Scott, T. W. Robinson, J. W. Tyler, G. C. Johnson, C. A. Carson, P. Cuddihee

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A 6-year-old Simmental cow infected with Theileria buffeli had a clinical disease characterized by theilerial parasitemia, macrocytic normochromic anemia with acanthocytosis and spherocytosis, lymphoid hyperplasia (lymphocytosis, edematous lymphadenomegaly), dysproteinemia, evidence of liver disease, and a low serum antibody titer against T. buffeli. The cow was in a herd in which all cattle originated in Missouri; 22/75 (29%) of cattle had a theilerial parasitemia and 26/75 (35%) had liters to T. buffeli of ≥1:160. Classification of the Missouri bovine organism as T. buffeli was based on DNA sequencing and comparison to sequences for T. buffeli and Theileria sp. type A obtained from GenBank. Intraerythrocytic veils and piroplasms were seen during transmission electron microscopy. The organism was successfully transmitted to two splenectomized calves, which developed mild anemias while parasitemic. Blood from the second calf was used as the source of T. buffeli antigen for an indirect immunofluorescence antibody test. Theilerial isolates from a Missouri white-tailed, deer were also sequenced and resembled Theileria sp. types F and G and were not consistent with the bovine organism.

Original languageEnglish (US)
Pages (from-to)11-21
Number of pages11
JournalVeterinary Pathology
Volume37
Issue number1
StatePublished - 2000

Fingerprint

Theileria buffeli
Theileriasis
Theileria
theileriosis
ultrastructure
beef
herds
case studies
Parasitemia
phylogeny
Abetalipoproteinemia
cattle
parasitemia
Macrocytic Anemia
Lymphocytosis
anemia
Deer
Viral Tumor Antigens
Nucleic Acid Databases
organisms

Keywords

  • Anemia
  • Cattle
  • Hemolysis
  • Nuclear small subunit ribosomal RNA gene
  • Phylogeny
  • Protozoa
  • Serology
  • Theileria
  • Theileriosis
  • Ultrastructure
  • White-tailed deer

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli : Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission. / Stockham, Steve L.; Kjemtrup, A. M.; Conrad, Patricia A; Schmidt, D. A.; Scott, M. A.; Robinson, T. W.; Tyler, J. W.; Johnson, G. C.; Carson, C. A.; Cuddihee, P.

In: Veterinary Pathology, Vol. 37, No. 1, 2000, p. 11-21.

Research output: Contribution to journalArticle

Stockham, SL, Kjemtrup, AM, Conrad, PA, Schmidt, DA, Scott, MA, Robinson, TW, Tyler, JW, Johnson, GC, Carson, CA & Cuddihee, P 2000, 'Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli: Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission', Veterinary Pathology, vol. 37, no. 1, pp. 11-21.
Stockham, Steve L. ; Kjemtrup, A. M. ; Conrad, Patricia A ; Schmidt, D. A. ; Scott, M. A. ; Robinson, T. W. ; Tyler, J. W. ; Johnson, G. C. ; Carson, C. A. ; Cuddihee, P. / Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli : Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission. In: Veterinary Pathology. 2000 ; Vol. 37, No. 1. pp. 11-21.
@article{aa1fee890f03437a83222f34b2bd0204,
title = "Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli: Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission",
abstract = "A 6-year-old Simmental cow infected with Theileria buffeli had a clinical disease characterized by theilerial parasitemia, macrocytic normochromic anemia with acanthocytosis and spherocytosis, lymphoid hyperplasia (lymphocytosis, edematous lymphadenomegaly), dysproteinemia, evidence of liver disease, and a low serum antibody titer against T. buffeli. The cow was in a herd in which all cattle originated in Missouri; 22/75 (29{\%}) of cattle had a theilerial parasitemia and 26/75 (35{\%}) had liters to T. buffeli of ≥1:160. Classification of the Missouri bovine organism as T. buffeli was based on DNA sequencing and comparison to sequences for T. buffeli and Theileria sp. type A obtained from GenBank. Intraerythrocytic veils and piroplasms were seen during transmission electron microscopy. The organism was successfully transmitted to two splenectomized calves, which developed mild anemias while parasitemic. Blood from the second calf was used as the source of T. buffeli antigen for an indirect immunofluorescence antibody test. Theilerial isolates from a Missouri white-tailed, deer were also sequenced and resembled Theileria sp. types F and G and were not consistent with the bovine organism.",
keywords = "Anemia, Cattle, Hemolysis, Nuclear small subunit ribosomal RNA gene, Phylogeny, Protozoa, Serology, Theileria, Theileriosis, Ultrastructure, White-tailed deer",
author = "Stockham, {Steve L.} and Kjemtrup, {A. M.} and Conrad, {Patricia A} and Schmidt, {D. A.} and Scott, {M. A.} and Robinson, {T. W.} and Tyler, {J. W.} and Johnson, {G. C.} and Carson, {C. A.} and P. Cuddihee",
year = "2000",
language = "English (US)",
volume = "37",
pages = "11--21",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "1",

}

TY - JOUR

T1 - Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli

T2 - Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission

AU - Stockham, Steve L.

AU - Kjemtrup, A. M.

AU - Conrad, Patricia A

AU - Schmidt, D. A.

AU - Scott, M. A.

AU - Robinson, T. W.

AU - Tyler, J. W.

AU - Johnson, G. C.

AU - Carson, C. A.

AU - Cuddihee, P.

PY - 2000

Y1 - 2000

N2 - A 6-year-old Simmental cow infected with Theileria buffeli had a clinical disease characterized by theilerial parasitemia, macrocytic normochromic anemia with acanthocytosis and spherocytosis, lymphoid hyperplasia (lymphocytosis, edematous lymphadenomegaly), dysproteinemia, evidence of liver disease, and a low serum antibody titer against T. buffeli. The cow was in a herd in which all cattle originated in Missouri; 22/75 (29%) of cattle had a theilerial parasitemia and 26/75 (35%) had liters to T. buffeli of ≥1:160. Classification of the Missouri bovine organism as T. buffeli was based on DNA sequencing and comparison to sequences for T. buffeli and Theileria sp. type A obtained from GenBank. Intraerythrocytic veils and piroplasms were seen during transmission electron microscopy. The organism was successfully transmitted to two splenectomized calves, which developed mild anemias while parasitemic. Blood from the second calf was used as the source of T. buffeli antigen for an indirect immunofluorescence antibody test. Theilerial isolates from a Missouri white-tailed, deer were also sequenced and resembled Theileria sp. types F and G and were not consistent with the bovine organism.

AB - A 6-year-old Simmental cow infected with Theileria buffeli had a clinical disease characterized by theilerial parasitemia, macrocytic normochromic anemia with acanthocytosis and spherocytosis, lymphoid hyperplasia (lymphocytosis, edematous lymphadenomegaly), dysproteinemia, evidence of liver disease, and a low serum antibody titer against T. buffeli. The cow was in a herd in which all cattle originated in Missouri; 22/75 (29%) of cattle had a theilerial parasitemia and 26/75 (35%) had liters to T. buffeli of ≥1:160. Classification of the Missouri bovine organism as T. buffeli was based on DNA sequencing and comparison to sequences for T. buffeli and Theileria sp. type A obtained from GenBank. Intraerythrocytic veils and piroplasms were seen during transmission electron microscopy. The organism was successfully transmitted to two splenectomized calves, which developed mild anemias while parasitemic. Blood from the second calf was used as the source of T. buffeli antigen for an indirect immunofluorescence antibody test. Theilerial isolates from a Missouri white-tailed, deer were also sequenced and resembled Theileria sp. types F and G and were not consistent with the bovine organism.

KW - Anemia

KW - Cattle

KW - Hemolysis

KW - Nuclear small subunit ribosomal RNA gene

KW - Phylogeny

KW - Protozoa

KW - Serology

KW - Theileria

KW - Theileriosis

KW - Ultrastructure

KW - White-tailed deer

UR - http://www.scopus.com/inward/record.url?scp=0033628185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033628185&partnerID=8YFLogxK

M3 - Article

C2 - 10643976

AN - SCOPUS:0033628185

VL - 37

SP - 11

EP - 21

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 1

ER -