Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli: Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission

Steve L. Stockham, A. M. Kjemtrup, Patricia A Conrad, D. A. Schmidt, M. A. Scott, T. W. Robinson, J. W. Tyler, G. C. Johnson, C. A. Carson, P. Cuddihee

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

A 6-year-old Simmental cow infected with Theileria buffeli had a clinical disease characterized by theilerial parasitemia, macrocytic normochromic anemia with acanthocytosis and spherocytosis, lymphoid hyperplasia (lymphocytosis, edematous lymphadenomegaly), dysproteinemia, evidence of liver disease, and a low serum antibody titer against T. buffeli. The cow was in a herd in which all cattle originated in Missouri; 22/75 (29%) of cattle had a theilerial parasitemia and 26/75 (35%) had liters to T. buffeli of ≥1:160. Classification of the Missouri bovine organism as T. buffeli was based on DNA sequencing and comparison to sequences for T. buffeli and Theileria sp. type A obtained from GenBank. Intraerythrocytic veils and piroplasms were seen during transmission electron microscopy. The organism was successfully transmitted to two splenectomized calves, which developed mild anemias while parasitemic. Blood from the second calf was used as the source of T. buffeli antigen for an indirect immunofluorescence antibody test. Theilerial isolates from a Missouri white-tailed, deer were also sequenced and resembled Theileria sp. types F and G and were not consistent with the bovine organism.

Original languageEnglish (US)
Pages (from-to)11-21
Number of pages11
JournalVeterinary Pathology
Volume37
Issue number1
StatePublished - 2000

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Keywords

  • Anemia
  • Cattle
  • Hemolysis
  • Nuclear small subunit ribosomal RNA gene
  • Phylogeny
  • Protozoa
  • Serology
  • Theileria
  • Theileriosis
  • Ultrastructure
  • White-tailed deer

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Stockham, S. L., Kjemtrup, A. M., Conrad, P. A., Schmidt, D. A., Scott, M. A., Robinson, T. W., Tyler, J. W., Johnson, G. C., Carson, C. A., & Cuddihee, P. (2000). Theileriosis in a Missouri Beef Herd Caused by Theileria buffeli: Case Report, Herd Investigation, Ultrastructure, Phylogenetic Analysis, and Experimental Transmission. Veterinary Pathology, 37(1), 11-21.