The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate

Sharon L. Amacher, Bruce W. Draper, Brian R. Summers, Charles B. Kimmel

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

T-box genes encode transcriptional regulators that control many aspects of embryonic development. Here, we demonstrate that the mesodermally expressed zebrafish spadetail (spt)/VegT and no tail (ntl)/Brachyury T-box genes are semi-redundantly and cell-autonomously required for formation of all trunk and tail mesoderm. Despite the lack of posterior mesoderm in spt-;ntl- embryos, dorsal-ventral neural tube patterning is relatively normal, with the notable exception that posterior medial floor plate is completely absent. This contrasts sharply with observations in single mutants, as mutations singly in ntl or spt enhance posterior medial floor plate development. We find that ntl function is required to repress medial floor plate and promote notochord fate in cells of the wild-type notochord domain and that spt and ntl together are required non cell-autonomously for medial floor plate formation, suggesting that an inducing signal present in wild-type mesoderm is lacking in spt-; ntl- embryos.

Original languageEnglish (US)
Pages (from-to)3311-3323
Number of pages13
JournalDevelopment
Volume129
Issue number14
StatePublished - Jul 2002

Fingerprint

Mesoderm
Zebrafish
Tail
Genes
Notochord
Embryonic Structures
Neural Tube
Embryonic Development
Mutation

Keywords

  • Brachyury
  • Fate map
  • Genetic mosaic
  • Genetic redundancy
  • Genetic synergy
  • VegT
  • Zebrafish

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Amacher, S. L., Draper, B. W., Summers, B. R., & Kimmel, C. B. (2002). The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development, 129(14), 3311-3323.

The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. / Amacher, Sharon L.; Draper, Bruce W.; Summers, Brian R.; Kimmel, Charles B.

In: Development, Vol. 129, No. 14, 07.2002, p. 3311-3323.

Research output: Contribution to journalArticle

Amacher, SL, Draper, BW, Summers, BR & Kimmel, CB 2002, 'The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate', Development, vol. 129, no. 14, pp. 3311-3323.
Amacher, Sharon L. ; Draper, Bruce W. ; Summers, Brian R. ; Kimmel, Charles B. / The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. In: Development. 2002 ; Vol. 129, No. 14. pp. 3311-3323.
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