The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3

Ping Zhou, Irina L. Derkatch, Susan M. Uptain, Maria M. Patino, Susan Lindquist, Susan W. Liebman

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The yeast non-Mendelian factor [ETA+] is lethal in the presence of certain mutations in the SUP35 and SUP45 genes, which code for the translational release factors eRF3 and eRF1, respectively. One such mutation, sup35-2, is now shown to contain a UAG stop codon prior to the essential region of the gene. The non-Mendelian inheritance of [ETA+] is reminiscent of the yeast [PSI+] element, which is due to a self-propagating conformation of Sup35p. Here we show that [ETA+] and [PSI+] share many characteristics. Indeed, like [PSI+], the maintenance of [ETA+] requires the N-terminal region of Sup35p and depends on an appropriate level of the chaperone protein Hsp104. Moreover, [ETA+] can be induced de novo by excess Sup35p, and [ETA+] cells have a weak nonsense suppressor phenotype characteristic of weak [PSI+]. We conclude that [ETA+] is actually a weak, unstable variant of [PSI+]. We find that although some Sup35p aggregates in [ETA+] cells, more Sup35p remains soluble in [ETA+] cells than in isogenic strong [PSI+] cells. Our data suggest that the amount of soluble Sup35p determines the strength of translational nonsense suppression associated with different [PSI+] variants.

Original languageEnglish (US)
Pages (from-to)1182-1191
Number of pages10
JournalEMBO Journal
Volume18
Issue number5
StatePublished - Mar 1 1999
Externally publishedYes

Fingerprint

Prions
Yeast
Genes
Yeasts
Conformations
Mutation
Terminator Codon
Essential Genes
Proteins
Maintenance
Phenotype

Keywords

  • [ETA]
  • [PSI]
  • Prion
  • Release factor
  • SUP35

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Zhou, P., Derkatch, I. L., Uptain, S. M., Patino, M. M., Lindquist, S., & Liebman, S. W. (1999). The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3. EMBO Journal, 18(5), 1182-1191.

The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3. / Zhou, Ping; Derkatch, Irina L.; Uptain, Susan M.; Patino, Maria M.; Lindquist, Susan; Liebman, Susan W.

In: EMBO Journal, Vol. 18, No. 5, 01.03.1999, p. 1182-1191.

Research output: Contribution to journalArticle

Zhou, P, Derkatch, IL, Uptain, SM, Patino, MM, Lindquist, S & Liebman, SW 1999, 'The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3', EMBO Journal, vol. 18, no. 5, pp. 1182-1191.
Zhou P, Derkatch IL, Uptain SM, Patino MM, Lindquist S, Liebman SW. The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3. EMBO Journal. 1999 Mar 1;18(5):1182-1191.
Zhou, Ping ; Derkatch, Irina L. ; Uptain, Susan M. ; Patino, Maria M. ; Lindquist, Susan ; Liebman, Susan W. / The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3. In: EMBO Journal. 1999 ; Vol. 18, No. 5. pp. 1182-1191.
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