Abstract
The structure of xylose reductase from Candida tenuis (AKR2B5) has been determined and refined to 2.2 Å resolution, both in holo and apo forms. These structures allow the recognition of numerous hydrophilic residues responsible for dimerization, a novel feature for the superfamily of enzymes. The residues allowing for dual NADH/NADPH cosubstrate specificity are also identified. Since xylose reductase functions in conjunction with an NAD+-specific xylitol dehydrogenase in the xylose assimilation pathway, this is a key step in engineering an enzyme specific for only NADH which will permit cosubstrate recycling between the two enzymes in a high-flux pathway. The structure of xylose reductase, combined with others in the superfamily provides an opportunity to examine and compare structural divergence as a function of sequence homology. It also suggests that the dimeric aldo-keto reductases (AKRs) from families 2 and 7 evolved from a common dimeric ancestor.
Original language | English (US) |
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Pages (from-to) | 515-521 |
Number of pages | 7 |
Journal | Chemico-Biological Interactions |
Volume | 143-144 |
DOIs | |
State | Published - Feb 1 2003 |
Keywords
- Candida tenuis
- Engineering
- Homology
ASJC Scopus subject areas
- Toxicology