The xylose reductase (AKR2B5) structure

Homology and divergence from other aldo-keto reductases and opportunities for protein engineering

David K. Wilson, Kathryn L. Kavanagh, Mario Klimacek, Bernd Nidetzky

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The structure of xylose reductase from Candida tenuis (AKR2B5) has been determined and refined to 2.2 Å resolution, both in holo and apo forms. These structures allow the recognition of numerous hydrophilic residues responsible for dimerization, a novel feature for the superfamily of enzymes. The residues allowing for dual NADH/NADPH cosubstrate specificity are also identified. Since xylose reductase functions in conjunction with an NAD+-specific xylitol dehydrogenase in the xylose assimilation pathway, this is a key step in engineering an enzyme specific for only NADH which will permit cosubstrate recycling between the two enzymes in a high-flux pathway. The structure of xylose reductase, combined with others in the superfamily provides an opportunity to examine and compare structural divergence as a function of sequence homology. It also suggests that the dimeric aldo-keto reductases (AKRs) from families 2 and 7 evolved from a common dimeric ancestor.

Original languageEnglish (US)
Pages (from-to)515-521
Number of pages7
JournalChemico-Biological Interactions
Volume143-144
DOIs
StatePublished - Feb 1 2003

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Protein Engineering
Aldehyde Reductase
NAD
D-Xylulose Reductase
Enzymes
Proteins
Dimerization
Candida
Xylose
Recycling
Sequence Homology
NADP
Fluxes
carbonyl reductase (NADPH)

Keywords

  • Candida tenuis
  • Engineering
  • Homology

ASJC Scopus subject areas

  • Toxicology

Cite this

The xylose reductase (AKR2B5) structure : Homology and divergence from other aldo-keto reductases and opportunities for protein engineering. / Wilson, David K.; Kavanagh, Kathryn L.; Klimacek, Mario; Nidetzky, Bernd.

In: Chemico-Biological Interactions, Vol. 143-144, 01.02.2003, p. 515-521.

Research output: Contribution to journalArticle

Wilson, David K. ; Kavanagh, Kathryn L. ; Klimacek, Mario ; Nidetzky, Bernd. / The xylose reductase (AKR2B5) structure : Homology and divergence from other aldo-keto reductases and opportunities for protein engineering. In: Chemico-Biological Interactions. 2003 ; Vol. 143-144. pp. 515-521.
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