The W-Beijing lineage of Mycobacterium tuberculosis overproduces triglycerides and has the DosR dormancy regulon constitutively upregulated

Michael B. Reed, Sebastien Gagneux, Kathryn DeRiemer, Peter M. Small, Clifton E. Barry

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

The Beijing family of Mycobacterium tuberculosis strains has been associated with epidemic spread and an increased likelihood of developing drug resistance. The characteristics that predispose this family to such clinical outcomes have not been identified, although one potential candidate, the phenolic glycolipid PGL-tb, has been shown to mediate a fulminant lethal disease in mice and rabbits due to lipid-mediated immunosuppression. However, PGL-tb is not uniformly expressed throughout the Beijing lineage and may not be the only unique virulence trait associated with this family. In an attempt to define phenotypes common to all Beijing strains, we interrogated a carefully selected set of isolates representing the five extant lineages of the Beijing family. Comparison of lipid production in this set revealed that all Beijing strains accumulated large quantities of triacylglycerides in in vitro aerobic culture. This accumulation was found to be coincident with upregulation of Rv3130c, whose product was previously characterized as a triacylglyceride synthase. Rv3130c is a member of the DosR-controlled regulon of M. tuberculosis, and further examination revealed that several members of this regulon were upregulated throughout this strain family. The upregulation of the DosR regulon may confer an adaptive advantage for growth in microaerophilic or anaerobic environments encountered by the bacillus during infection and thus may be related to the epidemiological phenomena associated with this important strain lineage.

Original languageEnglish (US)
Pages (from-to)2583-2589
Number of pages7
JournalJournal of Bacteriology
Volume189
Issue number7
DOIs
StatePublished - Apr 2007

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Regulon
Mycobacterium tuberculosis
Triglycerides
Up-Regulation
Lipids
Glycolipids
Drug Resistance
Immunosuppression
Bacillus
Virulence
Beijing
Rabbits
Phenotype
Growth
Infection

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The W-Beijing lineage of Mycobacterium tuberculosis overproduces triglycerides and has the DosR dormancy regulon constitutively upregulated. / Reed, Michael B.; Gagneux, Sebastien; DeRiemer, Kathryn; Small, Peter M.; Barry, Clifton E.

In: Journal of Bacteriology, Vol. 189, No. 7, 04.2007, p. 2583-2589.

Research output: Contribution to journalArticle

Reed, Michael B. ; Gagneux, Sebastien ; DeRiemer, Kathryn ; Small, Peter M. ; Barry, Clifton E. / The W-Beijing lineage of Mycobacterium tuberculosis overproduces triglycerides and has the DosR dormancy regulon constitutively upregulated. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 7. pp. 2583-2589.
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