The virB-encoded type IV secretion system is critical for establishment of infection and persistence of Brucella ovis infection in mice

Joicy C. Sá, Teane M A Silva, Érica A. Costa, Ana P C Silva, Renee M Tsolis, Tatiane A. Paixão, Alcina V. Carvalho Neta, Renato L. Santos

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Brucella spp. are gram-negative intracellular bacterial pathogens that cause chronic infections. Brucella virulence factors include a type IV secretion system (T4SS) and its lipopolysaccharide (LPS), which are essential for persistence. However, the role of the virB-encoded T4SS has not been investigated in naturally rough Brucella species such as Brucella ovis. In this study, male 6-week old BALBc mice were infected with B. ovis, Brucella abortus, and their respective Δ virB2 mutant strains. During early infection, B. ovis and B. abortus wild type strains were similarly recovered from spleen. Interestingly, in contrast to Δ virB2 B. abortus that was recovered at similar levels when compared to the wild type strain, the ΔvirB2 B. ovis was markedly attenuated as early as 24. h post infection (hpi). The Δ virB2 B. ovis was unable to survive and multiply in murine peritoneal macrophages and extracellularly within the peritoneal cavity at 12 and 24. hpi with lower splenic colonization than the parental strain at 6, 12 and 24. hpi. In contrast, wild type B. abortus and Δ virB2 B. abortus had a similar kinetics of infection in this model. As expected, the T4SS was essential for intracellular replication of smooth and rough strains in RAW macrophages at 48. hpi. These results suggest that T4SS is important for survival of B. ovis in murine model, and that a T4SS deficient B. ovis strain is cleared at earlier stages of infection when compared to a similar B. abortus mutant.

Original languageEnglish (US)
Pages (from-to)130-140
Number of pages11
JournalVeterinary Microbiology
Volume159
Issue number1-2
DOIs
StatePublished - Sep 14 2012

Keywords

  • Brucella ovis
  • LPS
  • Macrophage
  • Mouse
  • VirB

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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    Sá, J. C., Silva, T. M. A., Costa, É. A., Silva, A. P. C., Tsolis, R. M., Paixão, T. A., Carvalho Neta, A. V., & Santos, R. L. (2012). The virB-encoded type IV secretion system is critical for establishment of infection and persistence of Brucella ovis infection in mice. Veterinary Microbiology, 159(1-2), 130-140. https://doi.org/10.1016/j.vetmic.2012.03.029