The Vi Capsular Polysaccharide Enables Salmonella enterica Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis

Tamding Wangdi, Cheng Yuk Lee, Alanna M. Spees, Chenzhou Yu, Dawn D. Kingsbury, Sebastian E. Winter, Christine J. Hastey, R. Paul Wilson, Volkmar Heinrich, Andreas J Baumler

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever, a disseminated infection, while the closely related pathogen S. enterica serovar Typhimurium (S. Typhimurium) is associated with a localized gastroenteritis in humans. Here we investigated whether both pathogens differ in the chemotactic response they induce in neutrophils using a single-cell experimental approach. Surprisingly, neutrophils extended chemotactic pseudopodia toward Escherichia coli and S. Typhimurium, but not toward S. Typhi. Bacterial-guided chemotaxis was dependent on the presence of complement component 5a (C5a) and C5a receptor (C5aR). Deletion of S. Typhi capsule biosynthesis genes markedly enhanced the chemotactic response of neutrophils in vitro. Furthermore, deletion of capsule biosynthesis genes heightened the association of S. Typhi with neutrophils in vivo through a C5aR-dependent mechanism. Collectively, these data suggest that expression of the virulence-associated (Vi) capsular polysaccharide of S. Typhi obstructs bacterial-guided neutrophil chemotaxis.

Original languageEnglish (US)
JournalPLoS Pathogens
Volume10
Issue number8
DOIs
StatePublished - 2014

Fingerprint

Salmonella typhi
Chemotaxis
Polysaccharides
Virulence
Neutrophils
Capsules
Anaphylatoxin C5a Receptor
Complement C5a
Pseudopodia
Salmonella enterica
Typhoid Fever
Gastroenteritis
Genes
Serogroup
Escherichia coli
Infection

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

The Vi Capsular Polysaccharide Enables Salmonella enterica Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis. / Wangdi, Tamding; Lee, Cheng Yuk; Spees, Alanna M.; Yu, Chenzhou; Kingsbury, Dawn D.; Winter, Sebastian E.; Hastey, Christine J.; Wilson, R. Paul; Heinrich, Volkmar; Baumler, Andreas J.

In: PLoS Pathogens, Vol. 10, No. 8, 2014.

Research output: Contribution to journalArticle

Wangdi, T, Lee, CY, Spees, AM, Yu, C, Kingsbury, DD, Winter, SE, Hastey, CJ, Wilson, RP, Heinrich, V & Baumler, AJ 2014, 'The Vi Capsular Polysaccharide Enables Salmonella enterica Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis', PLoS Pathogens, vol. 10, no. 8. https://doi.org/10.1371/journal.ppat.1004306
Wangdi, Tamding ; Lee, Cheng Yuk ; Spees, Alanna M. ; Yu, Chenzhou ; Kingsbury, Dawn D. ; Winter, Sebastian E. ; Hastey, Christine J. ; Wilson, R. Paul ; Heinrich, Volkmar ; Baumler, Andreas J. / The Vi Capsular Polysaccharide Enables Salmonella enterica Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis. In: PLoS Pathogens. 2014 ; Vol. 10, No. 8.
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abstract = "Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever, a disseminated infection, while the closely related pathogen S. enterica serovar Typhimurium (S. Typhimurium) is associated with a localized gastroenteritis in humans. Here we investigated whether both pathogens differ in the chemotactic response they induce in neutrophils using a single-cell experimental approach. Surprisingly, neutrophils extended chemotactic pseudopodia toward Escherichia coli and S. Typhimurium, but not toward S. Typhi. Bacterial-guided chemotaxis was dependent on the presence of complement component 5a (C5a) and C5a receptor (C5aR). Deletion of S. Typhi capsule biosynthesis genes markedly enhanced the chemotactic response of neutrophils in vitro. Furthermore, deletion of capsule biosynthesis genes heightened the association of S. Typhi with neutrophils in vivo through a C5aR-dependent mechanism. Collectively, these data suggest that expression of the virulence-associated (Vi) capsular polysaccharide of S. Typhi obstructs bacterial-guided neutrophil chemotaxis.",
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