The use of the enzyme-linked immunosorbent assay (ELISA) in serological diagnosis of Mycoplasma bovis in dairy cattle

I. J. Uhaa, H. P. Riemann, Mark Thurmond, C. E. Franti

Research output: Contribution to journalArticle

13 Scopus citations


Between December 1985 and March 1987 an enzyme-linked immunosorbent assay (ELISA) was used with 3774 sera to estimate the prevalence of antibodies to Mycoplasma bovis in sera from three age groups of cattle in four dairies in California and to test for possible associations between the presence of M. bovis antibodies and the age or breed of the cattle and the farm. Unadjusted and adjusted associations were evaluated using the χ-square test for associations and multiple logistic regression analysis, respectively. There was a tendency for the proportion of cattle seropositive for M. bovis to increase steadily and approximately linearly with age (p<0.05). There was also a statistically significant relationship bbetween a M. bovis seropositive test and being from Farm IV (p<0.05). Farm IV was the largest of the four dairies and this association may be due to the effect of herd size. These findings confirm the ubiquitous distribution of antibodies to M. bovis in dairy cattle in California and also support previous reports of herd size as an important factor in mycoplasmal mastitis.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalVeterinary Research Communications
Issue number4
StatePublished - Jul 1 1990
Externally publishedYes



  • age
  • breed
  • California
  • cattle
  • Mycoplasma bovis
  • serodiagnosis

ASJC Scopus subject areas

  • veterinary(all)

Cite this