The use of synthetic Cecropin (D5C) in disinfecting contact lens solutions

L. B. Sousa, Mark J Mannis, I. R. Schwab, James S Cullor, H. Hosotani, W. Smith, J. Jaynes

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Purpose: Microbial keratitis caused by Pseudomonas aeruginosa is the most common contact lens associated corneal infection. Cecropins are microbicidal peptides isolated from the hemolymph of the Cecropia moth. Previous in vitro studies have demonstrated their efficacy against a broad spectrum of ocular pathogens. This study was designed: a) to evaluate the antimicrobial potency of three different contact lens solutions (Renu, Complete, and Opti-Free) against P. aeruginosa, and b) to evaluate the activity of the same contact lens solutions in combination with a synthetic cecropin analog, D5C, against the challenge organism in the presence of a soft contact lens. Methods: A virulent strain of P. aeruginosa isolated from a case of ulcerative keratitis was used in the study. Three different concentrations of bacteria (103, 105 and 107 CFU/mL) were inoculated into the contact lens solutions and into buffered saline, which was employed as a control. The samples were incubated at 27°C, and at time 0, 30, and 90 minutes, 24, 48, and 72 hours, and aliquots of the test solutions were plated and subcultured on nutrient agar. After 24 hours of incubation st 37°C, colonies observed on the nutrient agar plates were counted. To study the antimicrobial efficacy of D5C (100 μg/mL), we used the identical test series and assay, adding a soft contact lens to the solutions and a larger inoculum of bacteria (109 CFU/mL). Results: After 72 hours, all of the contact lens solutions tested sterilized 103 CFU/mL of P. aeruginosa. At 107 CFU/mL, they yielded greater than 2 logs of killing of the bacteria, but the solutions were not sterilized. The addition of D5C (100 μg/mL) to the contact lens solutions yielded greater than 3 logs of killing with a larger inoculum of bacteria in the presence of the soft contact lens. Conclusion: The contact lens solutions tested were effective against P. aeruginosa at 27°C for up to 72 hours with an inoculum of 103 CFU/mL. The addition of D5C augmented their antimicrobial activity in the presence of the contact lens.

Original languageEnglish (US)
Pages (from-to)114-117
Number of pages4
JournalCLAO Journal
Volume22
Issue number2
StatePublished - Apr 1996

ASJC Scopus subject areas

  • Ophthalmology

Fingerprint Dive into the research topics of 'The use of synthetic Cecropin (D<sub>5</sub>C) in disinfecting contact lens solutions'. Together they form a unique fingerprint.

  • Cite this

    Sousa, L. B., Mannis, M. J., Schwab, I. R., Cullor, J. S., Hosotani, H., Smith, W., & Jaynes, J. (1996). The use of synthetic Cecropin (D5C) in disinfecting contact lens solutions. CLAO Journal, 22(2), 114-117.