The use of optical tweezers to study sperm competition and motility in primates

Jaclyn M. Nascimento, Linda Z. Shi, Stuart A Meyers, Pascal Gagneux, Naida M. Loskutoff, Elliot L. Botvinick, Michael W. Berns

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Optical trapping is a non-invasive biophysical tool which has been widely applied to study physiological and biomechanical properties of cells. Using laser 'tweezers' in combination with custom-designed computer tracking algorithms, the swimming speeds and the relative swimming forces of individual sperm can be measured in real time. This combination of physical and engineering tools has been used to examine the evolutionary effect of sperm competition in primates. The results demonstrate a correlation between mating type and sperm motility: sperm from polygamous (multi-partner) primate species swim faster and with greater force than sperm from polygynous (single partner) primate species. In addition, sperm swimming force linearly increases with swimming speed for each species, yet the regression relating the two parameters is species specific. These results demonstrate the feasibility of using these tools to study rapidly moving (μms-1) biological cells.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalJournal of the Royal Society Interface
Volume5
Issue number20
DOIs
StatePublished - Mar 6 2008

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Keywords

  • Laser tweezers
  • Primate sperm competition
  • Sperm swimming force

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Biochemistry

Cite this

Nascimento, J. M., Shi, L. Z., Meyers, S. A., Gagneux, P., Loskutoff, N. M., Botvinick, E. L., & Berns, M. W. (2008). The use of optical tweezers to study sperm competition and motility in primates. Journal of the Royal Society Interface, 5(20), 297-302. https://doi.org/10.1098/rsif.2007.1118