The use of optical tweezers to study sperm competition and motility in primates

Jaclyn M. Nascimento, Linda Z. Shi, Stuart A Meyers, Pascal Gagneux, Naida M. Loskutoff, Elliot L. Botvinick, Michael W. Berns

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Optical trapping is a non-invasive biophysical tool which has been widely applied to study physiological and biomechanical properties of cells. Using laser 'tweezers' in combination with custom-designed computer tracking algorithms, the swimming speeds and the relative swimming forces of individual sperm can be measured in real time. This combination of physical and engineering tools has been used to examine the evolutionary effect of sperm competition in primates. The results demonstrate a correlation between mating type and sperm motility: sperm from polygamous (multi-partner) primate species swim faster and with greater force than sperm from polygynous (single partner) primate species. In addition, sperm swimming force linearly increases with swimming speed for each species, yet the regression relating the two parameters is species specific. These results demonstrate the feasibility of using these tools to study rapidly moving (μms-1) biological cells.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalJournal of the Royal Society Interface
Volume5
Issue number20
DOIs
StatePublished - Mar 6 2008

Fingerprint

Optical Tweezers
Optical tweezers
Sperm Motility
Primates
Spermatozoa
Swimming
Lasers

Keywords

  • Laser tweezers
  • Primate sperm competition
  • Sperm swimming force

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Biochemistry

Cite this

Nascimento, J. M., Shi, L. Z., Meyers, S. A., Gagneux, P., Loskutoff, N. M., Botvinick, E. L., & Berns, M. W. (2008). The use of optical tweezers to study sperm competition and motility in primates. Journal of the Royal Society Interface, 5(20), 297-302. https://doi.org/10.1098/rsif.2007.1118

The use of optical tweezers to study sperm competition and motility in primates. / Nascimento, Jaclyn M.; Shi, Linda Z.; Meyers, Stuart A; Gagneux, Pascal; Loskutoff, Naida M.; Botvinick, Elliot L.; Berns, Michael W.

In: Journal of the Royal Society Interface, Vol. 5, No. 20, 06.03.2008, p. 297-302.

Research output: Contribution to journalArticle

Nascimento, JM, Shi, LZ, Meyers, SA, Gagneux, P, Loskutoff, NM, Botvinick, EL & Berns, MW 2008, 'The use of optical tweezers to study sperm competition and motility in primates', Journal of the Royal Society Interface, vol. 5, no. 20, pp. 297-302. https://doi.org/10.1098/rsif.2007.1118
Nascimento, Jaclyn M. ; Shi, Linda Z. ; Meyers, Stuart A ; Gagneux, Pascal ; Loskutoff, Naida M. ; Botvinick, Elliot L. ; Berns, Michael W. / The use of optical tweezers to study sperm competition and motility in primates. In: Journal of the Royal Society Interface. 2008 ; Vol. 5, No. 20. pp. 297-302.
@article{4dc8fb3b1e1c4d59987a8ad80e1a5830,
title = "The use of optical tweezers to study sperm competition and motility in primates",
abstract = "Optical trapping is a non-invasive biophysical tool which has been widely applied to study physiological and biomechanical properties of cells. Using laser 'tweezers' in combination with custom-designed computer tracking algorithms, the swimming speeds and the relative swimming forces of individual sperm can be measured in real time. This combination of physical and engineering tools has been used to examine the evolutionary effect of sperm competition in primates. The results demonstrate a correlation between mating type and sperm motility: sperm from polygamous (multi-partner) primate species swim faster and with greater force than sperm from polygynous (single partner) primate species. In addition, sperm swimming force linearly increases with swimming speed for each species, yet the regression relating the two parameters is species specific. These results demonstrate the feasibility of using these tools to study rapidly moving (μms-1) biological cells.",
keywords = "Laser tweezers, Primate sperm competition, Sperm swimming force",
author = "Nascimento, {Jaclyn M.} and Shi, {Linda Z.} and Meyers, {Stuart A} and Pascal Gagneux and Loskutoff, {Naida M.} and Botvinick, {Elliot L.} and Berns, {Michael W.}",
year = "2008",
month = "3",
day = "6",
doi = "10.1098/rsif.2007.1118",
language = "English (US)",
volume = "5",
pages = "297--302",
journal = "Journal of the Royal Society Interface",
issn = "1742-5689",
publisher = "Royal Society of London",
number = "20",

}

TY - JOUR

T1 - The use of optical tweezers to study sperm competition and motility in primates

AU - Nascimento, Jaclyn M.

AU - Shi, Linda Z.

AU - Meyers, Stuart A

AU - Gagneux, Pascal

AU - Loskutoff, Naida M.

AU - Botvinick, Elliot L.

AU - Berns, Michael W.

PY - 2008/3/6

Y1 - 2008/3/6

N2 - Optical trapping is a non-invasive biophysical tool which has been widely applied to study physiological and biomechanical properties of cells. Using laser 'tweezers' in combination with custom-designed computer tracking algorithms, the swimming speeds and the relative swimming forces of individual sperm can be measured in real time. This combination of physical and engineering tools has been used to examine the evolutionary effect of sperm competition in primates. The results demonstrate a correlation between mating type and sperm motility: sperm from polygamous (multi-partner) primate species swim faster and with greater force than sperm from polygynous (single partner) primate species. In addition, sperm swimming force linearly increases with swimming speed for each species, yet the regression relating the two parameters is species specific. These results demonstrate the feasibility of using these tools to study rapidly moving (μms-1) biological cells.

AB - Optical trapping is a non-invasive biophysical tool which has been widely applied to study physiological and biomechanical properties of cells. Using laser 'tweezers' in combination with custom-designed computer tracking algorithms, the swimming speeds and the relative swimming forces of individual sperm can be measured in real time. This combination of physical and engineering tools has been used to examine the evolutionary effect of sperm competition in primates. The results demonstrate a correlation between mating type and sperm motility: sperm from polygamous (multi-partner) primate species swim faster and with greater force than sperm from polygynous (single partner) primate species. In addition, sperm swimming force linearly increases with swimming speed for each species, yet the regression relating the two parameters is species specific. These results demonstrate the feasibility of using these tools to study rapidly moving (μms-1) biological cells.

KW - Laser tweezers

KW - Primate sperm competition

KW - Sperm swimming force

UR - http://www.scopus.com/inward/record.url?scp=38349137710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349137710&partnerID=8YFLogxK

U2 - 10.1098/rsif.2007.1118

DO - 10.1098/rsif.2007.1118

M3 - Article

C2 - 17650470

AN - SCOPUS:38349137710

VL - 5

SP - 297

EP - 302

JO - Journal of the Royal Society Interface

JF - Journal of the Royal Society Interface

SN - 1742-5689

IS - 20

ER -