The use of one-bead one-compound combinatorial library method to identify peptide ligands for α4β1 integrin receptor in non-Hodgkin's lymphoma

Steven I. Park, Manat Renil, Brian Vikstrom, Nail Amro, Liang wen Song, Bai ling Xu, Kit Lam

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Using a 'one-bead one-compound' combinatorial library method and whole cell binding assay, we have identified peptide ligands that bind preferentially to human lymphoid malignant cells but not to normal human peripheral lymphocytes. These peptides have a common motif of Leu-Asp-Ile, Leu-Asp-Phe, or Leu-Asp-Val. Anti-α4 integrin antibody blocks binding of cells to these peptides which indicates that the target receptor for these peptides is α4β1 integrin. These findings may have important therapeutic and diagnostic implications in the treatment of lymphoma, leukemia, and various immunologic disorders.

Original languageEnglish (US)
Pages (from-to)171-178
Number of pages8
JournalLetters in Peptide Science
Volume8
Issue number3-5
DOIs
StatePublished - 2002

Fingerprint

Integrins
Non-Hodgkin's Lymphoma
Peptides
Ligands
Lymphocytes
Peptide Receptors
Assays
Lymphoma
Leukemia
Antibodies
Therapeutics

Keywords

  • Cancer therapy
  • Cell-adhesion
  • Combinatorial chemistry
  • Integrin
  • Non-Hodgkin lymphoma
  • Targeted-therapy

ASJC Scopus subject areas

  • Biochemistry

Cite this

The use of one-bead one-compound combinatorial library method to identify peptide ligands for α4β1 integrin receptor in non-Hodgkin's lymphoma. / Park, Steven I.; Renil, Manat; Vikstrom, Brian; Amro, Nail; Song, Liang wen; Xu, Bai ling; Lam, Kit.

In: Letters in Peptide Science, Vol. 8, No. 3-5, 2002, p. 171-178.

Research output: Contribution to journalArticle

Park, Steven I. ; Renil, Manat ; Vikstrom, Brian ; Amro, Nail ; Song, Liang wen ; Xu, Bai ling ; Lam, Kit. / The use of one-bead one-compound combinatorial library method to identify peptide ligands for α4β1 integrin receptor in non-Hodgkin's lymphoma. In: Letters in Peptide Science. 2002 ; Vol. 8, No. 3-5. pp. 171-178.
@article{9e841decf8d745569ef97bdcb34643e2,
title = "The use of one-bead one-compound combinatorial library method to identify peptide ligands for α4β1 integrin receptor in non-Hodgkin's lymphoma",
abstract = "Using a 'one-bead one-compound' combinatorial library method and whole cell binding assay, we have identified peptide ligands that bind preferentially to human lymphoid malignant cells but not to normal human peripheral lymphocytes. These peptides have a common motif of Leu-Asp-Ile, Leu-Asp-Phe, or Leu-Asp-Val. Anti-α4 integrin antibody blocks binding of cells to these peptides which indicates that the target receptor for these peptides is α4β1 integrin. These findings may have important therapeutic and diagnostic implications in the treatment of lymphoma, leukemia, and various immunologic disorders.",
keywords = "Cancer therapy, Cell-adhesion, Combinatorial chemistry, Integrin, Non-Hodgkin lymphoma, Targeted-therapy",
author = "Park, {Steven I.} and Manat Renil and Brian Vikstrom and Nail Amro and Song, {Liang wen} and Xu, {Bai ling} and Kit Lam",
year = "2002",
doi = "10.1023/A:1016297601361",
language = "English (US)",
volume = "8",
pages = "171--178",
journal = "International Journal of Peptide Research and Therapeutics",
issn = "1573-3149",
publisher = "Springer Netherlands",
number = "3-5",

}

TY - JOUR

T1 - The use of one-bead one-compound combinatorial library method to identify peptide ligands for α4β1 integrin receptor in non-Hodgkin's lymphoma

AU - Park, Steven I.

AU - Renil, Manat

AU - Vikstrom, Brian

AU - Amro, Nail

AU - Song, Liang wen

AU - Xu, Bai ling

AU - Lam, Kit

PY - 2002

Y1 - 2002

N2 - Using a 'one-bead one-compound' combinatorial library method and whole cell binding assay, we have identified peptide ligands that bind preferentially to human lymphoid malignant cells but not to normal human peripheral lymphocytes. These peptides have a common motif of Leu-Asp-Ile, Leu-Asp-Phe, or Leu-Asp-Val. Anti-α4 integrin antibody blocks binding of cells to these peptides which indicates that the target receptor for these peptides is α4β1 integrin. These findings may have important therapeutic and diagnostic implications in the treatment of lymphoma, leukemia, and various immunologic disorders.

AB - Using a 'one-bead one-compound' combinatorial library method and whole cell binding assay, we have identified peptide ligands that bind preferentially to human lymphoid malignant cells but not to normal human peripheral lymphocytes. These peptides have a common motif of Leu-Asp-Ile, Leu-Asp-Phe, or Leu-Asp-Val. Anti-α4 integrin antibody blocks binding of cells to these peptides which indicates that the target receptor for these peptides is α4β1 integrin. These findings may have important therapeutic and diagnostic implications in the treatment of lymphoma, leukemia, and various immunologic disorders.

KW - Cancer therapy

KW - Cell-adhesion

KW - Combinatorial chemistry

KW - Integrin

KW - Non-Hodgkin lymphoma

KW - Targeted-therapy

UR - http://www.scopus.com/inward/record.url?scp=0036330488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036330488&partnerID=8YFLogxK

U2 - 10.1023/A:1016297601361

DO - 10.1023/A:1016297601361

M3 - Article

VL - 8

SP - 171

EP - 178

JO - International Journal of Peptide Research and Therapeutics

JF - International Journal of Peptide Research and Therapeutics

SN - 1573-3149

IS - 3-5

ER -