The Use of Lectins in Bioaffinity MALDI Probes

Crystal Kirmiz, S. Caroline Chu, Carlito B Lebrilla

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Commonly, lectins have been utilized to isolate glycoproteins from biological fluids, such as serum and plasma. The use of lectins has advanced many areas of medical research, such as aging, cancer prevention, as well as other clinical problems. The chapter also desribes the work conducted in the Lebrilla Laboratory utilizing lectin biology to isolate glycans present in the egg jelly of the frog Xenopus laevis. In this project, the general use of bioaffinity probes for oligosaccharide analysis was examined prior to the work of developing bioaffinity matrix-assisted laser desorption/ionization (MALDI) lectin probes. The methods developed in this phase of the project were ultimately used with the lectins to extract glycans from complex and heterogenous mixtures. Biologically active probes for MALDI can be highly useful for the rapid isolation of a compound from a mixture, thus allowing researchers to complete rapid analysis using mass spectrometry (MS).

Original languageEnglish (US)
Title of host publicationLectins: Analytical Technologies
PublisherElsevier
Pages327-342
Number of pages16
ISBN (Print)9780444530776
DOIs
StatePublished - 2007

Fingerprint

Lectins
Ionization
Desorption
Lasers
Polysaccharides
Oligosaccharides
Mass spectrometry
Glycoproteins
Aging of materials
Plasmas
Fluids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Kirmiz, C., Chu, S. C., & Lebrilla, C. B. (2007). The Use of Lectins in Bioaffinity MALDI Probes. In Lectins: Analytical Technologies (pp. 327-342). Elsevier. https://doi.org/10.1016/B978-044453077-6/50014-4

The Use of Lectins in Bioaffinity MALDI Probes. / Kirmiz, Crystal; Chu, S. Caroline; Lebrilla, Carlito B.

Lectins: Analytical Technologies. Elsevier, 2007. p. 327-342.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kirmiz, C, Chu, SC & Lebrilla, CB 2007, The Use of Lectins in Bioaffinity MALDI Probes. in Lectins: Analytical Technologies. Elsevier, pp. 327-342. https://doi.org/10.1016/B978-044453077-6/50014-4
Kirmiz C, Chu SC, Lebrilla CB. The Use of Lectins in Bioaffinity MALDI Probes. In Lectins: Analytical Technologies. Elsevier. 2007. p. 327-342 https://doi.org/10.1016/B978-044453077-6/50014-4
Kirmiz, Crystal ; Chu, S. Caroline ; Lebrilla, Carlito B. / The Use of Lectins in Bioaffinity MALDI Probes. Lectins: Analytical Technologies. Elsevier, 2007. pp. 327-342
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