The use of enzyme-linked immunosorbent assays (ELISA) for the determination of Triton X nonionic detergents

Siong I. Wie, Bruce D. Hammock

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-permilliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.

Original languageEnglish (US)
Pages (from-to)168-176
Number of pages9
JournalAnalytical Biochemistry
Volume125
Issue number1
DOIs
StatePublished - Sep 1 1982

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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