Abstract
An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-permilliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.
Original language | English (US) |
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Pages (from-to) | 168-176 |
Number of pages | 9 |
Journal | Analytical Biochemistry |
Volume | 125 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1 1982 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology