Abstract
An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-permilliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 168-176 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 125 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 1 1982 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Fingerprint Dive into the research topics of 'The use of enzyme-linked immunosorbent assays (ELISA) for the determination of Triton X nonionic detergents'. Together they form a unique fingerprint.
Cite this
Wie, S. I., & Hammock, B. D. (1982). The use of enzyme-linked immunosorbent assays (ELISA) for the determination of Triton X nonionic detergents. Analytical Biochemistry, 125(1), 168-176. https://doi.org/10.1016/0003-2697(82)90399-2