The use of class-I HLA tetramers for the detection of hepatitis C virus NS3-specific CD8+ T cells in patients with chronic infection

F. Xavier López-Labrador, Xiaosong He, Marina Berenguer, Ramsey C. Cheung, Teresa L. Wright, Harry B. Greenberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background/aims: New methods to detect virus-specific T-cell responses have recently been developed. Several human leukocyte antigen (HLA)-peptide tetramers for the detection of hepatitis C virus (HCV)-specific CD8+ T cells are under evaluation. Methods: Evaluation of one HLA class I-tetramer (HCVNS3-2) for the detection of HCV NS3-specific CD8+ T cells in a series of 38 HLA-A2+ chronically infected patients. Results: Almost half (42%) of the patients had detectable NS3-specific CD8+ T cells. The frequencies of such cells ranged from 0.01% to 0.22% of total CD8+ T cells. No significant differences in clinical features or mean viral load were detected between patients with or without tetramer+CD8+ T cells. Conclusions: The tetramer HCVNS3-2 may be very useful for the study of the HCV-specific CD8+ immune response. Combination of this reagent with other tetramers based on other HCV peptides may help in the understanding of the immune response to the virus. However, a panel of tetramers based on several parts of the HCV polyprotein may be a mandatory requirement to explore the whole breadth of the CD8+ T-cell response against HCV and to detect that response in the majority of patients with chronic infection.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalJournal of Immunological Methods
Volume287
Issue number1-2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

HLA Antigens
Hepacivirus
T-Lymphocytes
Infection
Viruses
Polyproteins
Peptides
Viral Load
varespladib methyl

Keywords

  • Alanine aminotransferase
  • ALT
  • Cytotoxic T cells
  • Epitope
  • HBsAg
  • HCV
  • HCV non-structural protein 3
  • Hepatitis B surface antigen
  • Hepatitis C virus
  • HIV
  • HLA
  • Human immunodeficiency virus
  • Human leukocyte antigen
  • Immunotherapy
  • NS3
  • T-cell receptor
  • Viral immunity

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

The use of class-I HLA tetramers for the detection of hepatitis C virus NS3-specific CD8+ T cells in patients with chronic infection. / López-Labrador, F. Xavier; He, Xiaosong; Berenguer, Marina; Cheung, Ramsey C.; Wright, Teresa L.; Greenberg, Harry B.

In: Journal of Immunological Methods, Vol. 287, No. 1-2, 04.2004, p. 91-99.

Research output: Contribution to journalArticle

López-Labrador, F. Xavier ; He, Xiaosong ; Berenguer, Marina ; Cheung, Ramsey C. ; Wright, Teresa L. ; Greenberg, Harry B. / The use of class-I HLA tetramers for the detection of hepatitis C virus NS3-specific CD8+ T cells in patients with chronic infection. In: Journal of Immunological Methods. 2004 ; Vol. 287, No. 1-2. pp. 91-99.
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abstract = "Background/aims: New methods to detect virus-specific T-cell responses have recently been developed. Several human leukocyte antigen (HLA)-peptide tetramers for the detection of hepatitis C virus (HCV)-specific CD8+ T cells are under evaluation. Methods: Evaluation of one HLA class I-tetramer (HCVNS3-2) for the detection of HCV NS3-specific CD8+ T cells in a series of 38 HLA-A2+ chronically infected patients. Results: Almost half (42{\%}) of the patients had detectable NS3-specific CD8+ T cells. The frequencies of such cells ranged from 0.01{\%} to 0.22{\%} of total CD8+ T cells. No significant differences in clinical features or mean viral load were detected between patients with or without tetramer+CD8+ T cells. Conclusions: The tetramer HCVNS3-2 may be very useful for the study of the HCV-specific CD8+ immune response. Combination of this reagent with other tetramers based on other HCV peptides may help in the understanding of the immune response to the virus. However, a panel of tetramers based on several parts of the HCV polyprotein may be a mandatory requirement to explore the whole breadth of the CD8+ T-cell response against HCV and to detect that response in the majority of patients with chronic infection.",
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AB - Background/aims: New methods to detect virus-specific T-cell responses have recently been developed. Several human leukocyte antigen (HLA)-peptide tetramers for the detection of hepatitis C virus (HCV)-specific CD8+ T cells are under evaluation. Methods: Evaluation of one HLA class I-tetramer (HCVNS3-2) for the detection of HCV NS3-specific CD8+ T cells in a series of 38 HLA-A2+ chronically infected patients. Results: Almost half (42%) of the patients had detectable NS3-specific CD8+ T cells. The frequencies of such cells ranged from 0.01% to 0.22% of total CD8+ T cells. No significant differences in clinical features or mean viral load were detected between patients with or without tetramer+CD8+ T cells. Conclusions: The tetramer HCVNS3-2 may be very useful for the study of the HCV-specific CD8+ immune response. Combination of this reagent with other tetramers based on other HCV peptides may help in the understanding of the immune response to the virus. However, a panel of tetramers based on several parts of the HCV polyprotein may be a mandatory requirement to explore the whole breadth of the CD8+ T-cell response against HCV and to detect that response in the majority of patients with chronic infection.

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