Abstract
A new method for the measurement of serum vitamin B12 concentration by radioisotope dilution is described in which chicken serum is used as the source of vitamin B12 binding protein. The conditions for the assay were carefully selected on the basis of a thorough investigation of the factors affecting the critical steps in the radioisotope dilution assay for vitamin B12. The assay obeys the principle of radioisotope dilution, and gives reproducible results. The normal range for the method (400 to 1020 pg/ml) is higher than any previously reported normal range for a radioisotope method. Excellent discrimination was obtained between normal subjects and those with pernicious anemia. The serum vitamin B12 levels measured by the chicken serum radioisotope dilution assay in 596 subjects were compared with the results of either Euglena gracilis or Lactobacillus leichmannii microbiological assay. The chicken serum method consistently gave higher results than either microbiological assay, but the difference was especially great in many post gastrectomy, pregnancy, polycythemia vera and cord sera. A definite cause for these differences was not established.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 507-526 |
| Number of pages | 20 |
| Journal | British Journal of Haematology |
| Volume | 27 |
| Issue number | 3 |
| State | Published - 1974 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Hematology
Cite this
The use of chicken serum for measurement of serum vitamin B12 concentration by radioisotope dilution : description of method and comparison with microbiological assay results. / Green, Ralph; Newmark, P. A.; Musso, A. M.; Mollin, D. L.
In: British Journal of Haematology, Vol. 27, No. 3, 1974, p. 507-526.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The use of chicken serum for measurement of serum vitamin B12 concentration by radioisotope dilution
T2 - description of method and comparison with microbiological assay results
AU - Green, Ralph
AU - Newmark, P. A.
AU - Musso, A. M.
AU - Mollin, D. L.
PY - 1974
Y1 - 1974
N2 - A new method for the measurement of serum vitamin B12 concentration by radioisotope dilution is described in which chicken serum is used as the source of vitamin B12 binding protein. The conditions for the assay were carefully selected on the basis of a thorough investigation of the factors affecting the critical steps in the radioisotope dilution assay for vitamin B12. The assay obeys the principle of radioisotope dilution, and gives reproducible results. The normal range for the method (400 to 1020 pg/ml) is higher than any previously reported normal range for a radioisotope method. Excellent discrimination was obtained between normal subjects and those with pernicious anemia. The serum vitamin B12 levels measured by the chicken serum radioisotope dilution assay in 596 subjects were compared with the results of either Euglena gracilis or Lactobacillus leichmannii microbiological assay. The chicken serum method consistently gave higher results than either microbiological assay, but the difference was especially great in many post gastrectomy, pregnancy, polycythemia vera and cord sera. A definite cause for these differences was not established.
AB - A new method for the measurement of serum vitamin B12 concentration by radioisotope dilution is described in which chicken serum is used as the source of vitamin B12 binding protein. The conditions for the assay were carefully selected on the basis of a thorough investigation of the factors affecting the critical steps in the radioisotope dilution assay for vitamin B12. The assay obeys the principle of radioisotope dilution, and gives reproducible results. The normal range for the method (400 to 1020 pg/ml) is higher than any previously reported normal range for a radioisotope method. Excellent discrimination was obtained between normal subjects and those with pernicious anemia. The serum vitamin B12 levels measured by the chicken serum radioisotope dilution assay in 596 subjects were compared with the results of either Euglena gracilis or Lactobacillus leichmannii microbiological assay. The chicken serum method consistently gave higher results than either microbiological assay, but the difference was especially great in many post gastrectomy, pregnancy, polycythemia vera and cord sera. A definite cause for these differences was not established.
UR - http://www.scopus.com/inward/record.url?scp=0016204717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016204717&partnerID=8YFLogxK
M3 - Article
VL - 27
SP - 507
EP - 526
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 3
ER -