Abstract
Centrins are a ubiquitous family of small Ca2+-binding proteins found at basal bodies that are placed into two groups based on sequence similarity to the human centrins 2 and 3. Analyses of basal body composition in different species suggest that they contain a centrin isoform from each group. We used the ciliate protist Tetrahymena thermophila to gain a better understanding of the functions of the two centrin groups and to determine their potential redundancy. We have previously shown that the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is required for proper basal body function. In this paper, we show that the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has functions similar to Cen1 in basal body orientation, maintenance, and separation. The two are, how ever, not redundant. A further examination of human centrin 3 homologues shows that they function in a manner distinct from human centrin 2 homologues. Our data suggest that basal bodies require a centrin from both groups in order to function correctly.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4766-4777 |
| Number of pages | 12 |
| Journal | Molecular Biology of the Cell |
| Volume | 23 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 15 2012 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
Cite this
The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies. / Vonderfecht, Tyson; Cookson, Michael W.; Giddings, Thomas H.; Clarissa, Christina; Winey, Mark.
In: Molecular Biology of the Cell, Vol. 23, No. 24, 15.12.2012, p. 4766-4777.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies
AU - Vonderfecht, Tyson
AU - Cookson, Michael W.
AU - Giddings, Thomas H.
AU - Clarissa, Christina
AU - Winey, Mark
PY - 2012/12/15
Y1 - 2012/12/15
N2 - Centrins are a ubiquitous family of small Ca2+-binding proteins found at basal bodies that are placed into two groups based on sequence similarity to the human centrins 2 and 3. Analyses of basal body composition in different species suggest that they contain a centrin isoform from each group. We used the ciliate protist Tetrahymena thermophila to gain a better understanding of the functions of the two centrin groups and to determine their potential redundancy. We have previously shown that the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is required for proper basal body function. In this paper, we show that the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has functions similar to Cen1 in basal body orientation, maintenance, and separation. The two are, how ever, not redundant. A further examination of human centrin 3 homologues shows that they function in a manner distinct from human centrin 2 homologues. Our data suggest that basal bodies require a centrin from both groups in order to function correctly.
AB - Centrins are a ubiquitous family of small Ca2+-binding proteins found at basal bodies that are placed into two groups based on sequence similarity to the human centrins 2 and 3. Analyses of basal body composition in different species suggest that they contain a centrin isoform from each group. We used the ciliate protist Tetrahymena thermophila to gain a better understanding of the functions of the two centrin groups and to determine their potential redundancy. We have previously shown that the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is required for proper basal body function. In this paper, we show that the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has functions similar to Cen1 in basal body orientation, maintenance, and separation. The two are, how ever, not redundant. A further examination of human centrin 3 homologues shows that they function in a manner distinct from human centrin 2 homologues. Our data suggest that basal bodies require a centrin from both groups in order to function correctly.
UR - http://www.scopus.com/inward/record.url?scp=84871234016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871234016&partnerID=8YFLogxK
U2 - 10.1091/mbc.E12-06-0454
DO - 10.1091/mbc.E12-06-0454
M3 - Article
C2 - 23087207
AN - SCOPUS:84871234016
VL - 23
SP - 4766
EP - 4777
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 24
ER -