The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies

Tyson Vonderfecht, Michael W. Cookson, Thomas H. Giddings, Christina Clarissa, Mark Winey

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Centrins are a ubiquitous family of small Ca2+-binding proteins found at basal bodies that are placed into two groups based on sequence similarity to the human centrins 2 and 3. Analyses of basal body composition in different species suggest that they contain a centrin isoform from each group. We used the ciliate protist Tetrahymena thermophila to gain a better understanding of the functions of the two centrin groups and to determine their potential redundancy. We have previously shown that the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is required for proper basal body function. In this paper, we show that the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has functions similar to Cen1 in basal body orientation, maintenance, and separation. The two are, how ever, not redundant. A further examination of human centrin 3 homologues shows that they function in a manner distinct from human centrin 2 homologues. Our data suggest that basal bodies require a centrin from both groups in order to function correctly.

Original languageEnglish (US)
Pages (from-to)4766-4777
Number of pages12
JournalMolecular Biology of the Cell
Volume23
Issue number24
DOIs
StatePublished - Dec 15 2012
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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