The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity

Sebastian E. Winter, Maria G. Winter, Parameth Thiennimitr, Valerie A. Gerriets, Sean Paul Nuccio, Holger Rüssmann, Andreas J Baumler

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/ RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasionassociated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion, reduced motility and reduced expression of FliC. Both RcsB and TviA repressed expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. Introduction of tviA into S. enterica serotype Typhimurium rendered flhDC transcription sensitive to changes in osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity.

Original languageEnglish (US)
Pages (from-to)175-193
Number of pages19
JournalMolecular Microbiology
Volume74
Issue number1
DOIs
StatePublished - Oct 2009

Fingerprint

Regulon
Osmolar Concentration
Salmonella enterica
Flagella
Gene Expression
Proteins
Osmoregulation
Antigens
Gene Expression Profiling
Capsules
Virulence
Serogroup
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity. / Winter, Sebastian E.; Winter, Maria G.; Thiennimitr, Parameth; Gerriets, Valerie A.; Nuccio, Sean Paul; Rüssmann, Holger; Baumler, Andreas J.

In: Molecular Microbiology, Vol. 74, No. 1, 10.2009, p. 175-193.

Research output: Contribution to journalArticle

Winter, Sebastian E. ; Winter, Maria G. ; Thiennimitr, Parameth ; Gerriets, Valerie A. ; Nuccio, Sean Paul ; Rüssmann, Holger ; Baumler, Andreas J. / The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity. In: Molecular Microbiology. 2009 ; Vol. 74, No. 1. pp. 175-193.
@article{9b1ff7f10bac40bf8b92d4acbaf1c4db,
title = "The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity",
abstract = "In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/ RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasionassociated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion, reduced motility and reduced expression of FliC. Both RcsB and TviA repressed expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. Introduction of tviA into S. enterica serotype Typhimurium rendered flhDC transcription sensitive to changes in osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity.",
author = "Winter, {Sebastian E.} and Winter, {Maria G.} and Parameth Thiennimitr and Gerriets, {Valerie A.} and Nuccio, {Sean Paul} and Holger R{\"u}ssmann and Baumler, {Andreas J}",
year = "2009",
month = "10",
doi = "10.1111/j.1365-2958.2009.06859.x",
language = "English (US)",
volume = "74",
pages = "175--193",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity

AU - Winter, Sebastian E.

AU - Winter, Maria G.

AU - Thiennimitr, Parameth

AU - Gerriets, Valerie A.

AU - Nuccio, Sean Paul

AU - Rüssmann, Holger

AU - Baumler, Andreas J

PY - 2009/10

Y1 - 2009/10

N2 - In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/ RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasionassociated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion, reduced motility and reduced expression of FliC. Both RcsB and TviA repressed expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. Introduction of tviA into S. enterica serotype Typhimurium rendered flhDC transcription sensitive to changes in osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity.

AB - In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/ RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasionassociated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion, reduced motility and reduced expression of FliC. Both RcsB and TviA repressed expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. Introduction of tviA into S. enterica serotype Typhimurium rendered flhDC transcription sensitive to changes in osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity.

UR - http://www.scopus.com/inward/record.url?scp=70350169211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350169211&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2958.2009.06859.x

DO - 10.1111/j.1365-2958.2009.06859.x

M3 - Article

C2 - 19703107

AN - SCOPUS:70350169211

VL - 74

SP - 175

EP - 193

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 1

ER -