The tricarboxylic acid cycle in Dictyostelium discoideum: I. Formulation of alternative kinetic representations

Fumihide Shiraishi, Michael A. Savageau

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Enzyme systems within living cells have recently been shown to be highly ordered structures that violate classic assumptions of the Michaelis-Menten formalism, which originally was developed for the characterization of isolated reactions in vitro. This evidence suggests that a thorough examination of alternative kinetic formalisms for integrated biochemical systems is in order. The purpose of this series of papers is to assess the utility of an alternative power-law formalism by carrying out a detailed comparative analysis of a relatively large, representative system - the tricarboxylic acid cycle of Dictyostelium discoideum. This system was chosen because considerable experimental information already has been synthesized into a detailed kinetic model of the intact system. In this first paper, we set the stage for subsequent analysis within the framework of the power-law formalism: we review the underlying theory, emphasizing recent developments, formulate the model in terms that are convenient for the analysis to follow, and develop the system representation in both the Michaelis-Menten and power-law forms. In the second paper (Shiraishi, F., and Savageau, M. A. (1992) J. Biol. Chem. 267, 22919-22925), these alternative representations are shown to be internally consistent and locally equivalent. The third paper (Shiraishi, F., and Savageau, M. A. (1992) J. Biol. Chem. 267, 22926-22933) provides a complete analysis of the steady state behavior and also treats the dynamic behavior of the model.

Original languageEnglish (US)
Pages (from-to)22912-22918
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number32
StatePublished - Nov 15 1992
Externally publishedYes

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Dictyostelium
Citric Acid Cycle
Kinetics
Enzymes
Cells
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

The tricarboxylic acid cycle in Dictyostelium discoideum : I. Formulation of alternative kinetic representations. / Shiraishi, Fumihide; Savageau, Michael A.

In: Journal of Biological Chemistry, Vol. 267, No. 32, 15.11.1992, p. 22912-22918.

Research output: Contribution to journalArticle

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