The tributyltin leads to obesogenic mammary gland abnormalities in adult female rats

Charles S. da Costa, Leandro Miranda-Alves, Michele La Merrill, Ian V. Silva, Jones B. Graceli

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Tributyltin chloride (TBT) is an obesogen associated with several complications. However, few investigations have evaluated TBT effects on adult mammary glands (MG). In this investigation, we assessed whether TBT's obesogenic effects resulted in abnormal MG fat pad expansion and other irregularities. TBT was administered to female rats (100 ng/kg/day for 15 days via gavage), and their MG morphophysiological development was assessed. We further assessed the MG fat pad for PPARγ, ERα, and aromatase protein expression, as well as inflammation, oxidative stress (OS), apoptosis and fibrosis. Irregular MG morphological development such as lower TEB number, alveolar (AB), lobule and differentiation (DF) score were observed in TBT rats. TBT rats had abnormal MG fat accumulation as evidenced by increased numbers of hypertrophic adipocytes, triglyceride (TG) levels and PPARγ expression. A strong negative correlation between the MG obesogenic makers and TEB number, AB and DF score were observed in TBT rats. MG inflammation was observed in TBT rats. A positive correlation between the MG obesogenic markers and inflammation were observed. High ERα and aromatase expression were observed in MG of TBT rats. MG OS, apoptosis and fibrosis were present in the TBT rats. Additionally, a positive correlation between the MG obesogenic markers and OS were observed in TBT rats. Thus, these data suggest that obesogenic TBT effects led to MG irregularities in the adult female rats.

Original languageEnglish (US)
Pages (from-to)59-71
Number of pages13
JournalToxicology Letters
Volume307
DOIs
StatePublished - Jun 1 2019

Keywords

  • Endocrine-disrupting chemicals
  • ERα/aromatase
  • Inflammation
  • Mammary gland fat pad
  • Oxidative stress
  • Tributyltin chloride

ASJC Scopus subject areas

  • Toxicology

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