The transcriptome of estrogen-independent mammary growth in female mice reveals that not all mammary glands are created equally

Grace E. Berryhill, Danielle G. Lemay, Josephine F. Trott, Lucila Aimo, Adam L. Lock, Russell C. Hovey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Allometric growth of ducts in the mammary glands (MGs) is widely held to be estrogen dependent. We previously discovered that the dietary fatty acid trans-10, cis-12 conjugated linoleic acid (CLA) stimulates estrogen-independent allometric growth and terminal end bud formation in ovariectomized mice. Given the similar phenotype induced by estrogen and CLA, we investigated the shared and/or divergent mechanisms underlying these changes. We confirmed MG growth induced by CLA is temporally distinct from that elicited by estrogen. We then used RNA sequencing to compare the transcriptome of the MG during similar proliferative and morphological states. Both estrogen and CLA affected the genes involved in proliferation. The transcriptome for estrogentreated mice included canonical estrogen-induced genes, including Pgr, Areg, and Foxa1. In contrast, their expression was unchanged by CLA. However, CLA, but not estrogen, altered expression of a unique set of inflammation-Associated genes, consistent with stromal changes. This CLA-Altered signature included increased expression of epidermal growth factor receptor (EGFR) pathway components, consistent with the demonstration that CLA-induced MG growth is EGFR dependent. Our findings highlight a unique role for diet-induced inflammation that underlies estrogenindependent MG development. (Endocrinology 158: 3126-3139, 2017).

Original languageEnglish (US)
Pages (from-to)3126-3139
Number of pages14
JournalEndocrinology
Volume158
Issue number10
DOIs
StatePublished - Oct 1 2017

Fingerprint

Conjugated Linoleic Acids
Human Mammary Glands
Transcriptome
Estrogens
Breast
Growth
Epidermal Growth Factor Receptor
Genes
Inflammation
RNA Sequence Analysis
Endocrinology
Fatty Acids
Diet
Phenotype

ASJC Scopus subject areas

  • Endocrinology

Cite this

The transcriptome of estrogen-independent mammary growth in female mice reveals that not all mammary glands are created equally. / Berryhill, Grace E.; Lemay, Danielle G.; Trott, Josephine F.; Aimo, Lucila; Lock, Adam L.; Hovey, Russell C.

In: Endocrinology, Vol. 158, No. 10, 01.10.2017, p. 3126-3139.

Research output: Contribution to journalArticle

Berryhill, Grace E. ; Lemay, Danielle G. ; Trott, Josephine F. ; Aimo, Lucila ; Lock, Adam L. ; Hovey, Russell C. / The transcriptome of estrogen-independent mammary growth in female mice reveals that not all mammary glands are created equally. In: Endocrinology. 2017 ; Vol. 158, No. 10. pp. 3126-3139.
@article{563b5b490f1d48b48ae276531f22bb8d,
title = "The transcriptome of estrogen-independent mammary growth in female mice reveals that not all mammary glands are created equally",
abstract = "Allometric growth of ducts in the mammary glands (MGs) is widely held to be estrogen dependent. We previously discovered that the dietary fatty acid trans-10, cis-12 conjugated linoleic acid (CLA) stimulates estrogen-independent allometric growth and terminal end bud formation in ovariectomized mice. Given the similar phenotype induced by estrogen and CLA, we investigated the shared and/or divergent mechanisms underlying these changes. We confirmed MG growth induced by CLA is temporally distinct from that elicited by estrogen. We then used RNA sequencing to compare the transcriptome of the MG during similar proliferative and morphological states. Both estrogen and CLA affected the genes involved in proliferation. The transcriptome for estrogentreated mice included canonical estrogen-induced genes, including Pgr, Areg, and Foxa1. In contrast, their expression was unchanged by CLA. However, CLA, but not estrogen, altered expression of a unique set of inflammation-Associated genes, consistent with stromal changes. This CLA-Altered signature included increased expression of epidermal growth factor receptor (EGFR) pathway components, consistent with the demonstration that CLA-induced MG growth is EGFR dependent. Our findings highlight a unique role for diet-induced inflammation that underlies estrogenindependent MG development. (Endocrinology 158: 3126-3139, 2017).",
author = "Berryhill, {Grace E.} and Lemay, {Danielle G.} and Trott, {Josephine F.} and Lucila Aimo and Lock, {Adam L.} and Hovey, {Russell C.}",
year = "2017",
month = "10",
day = "1",
doi = "10.1210/en.2017-00395",
language = "English (US)",
volume = "158",
pages = "3126--3139",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "10",

}

TY - JOUR

T1 - The transcriptome of estrogen-independent mammary growth in female mice reveals that not all mammary glands are created equally

AU - Berryhill, Grace E.

AU - Lemay, Danielle G.

AU - Trott, Josephine F.

AU - Aimo, Lucila

AU - Lock, Adam L.

AU - Hovey, Russell C.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Allometric growth of ducts in the mammary glands (MGs) is widely held to be estrogen dependent. We previously discovered that the dietary fatty acid trans-10, cis-12 conjugated linoleic acid (CLA) stimulates estrogen-independent allometric growth and terminal end bud formation in ovariectomized mice. Given the similar phenotype induced by estrogen and CLA, we investigated the shared and/or divergent mechanisms underlying these changes. We confirmed MG growth induced by CLA is temporally distinct from that elicited by estrogen. We then used RNA sequencing to compare the transcriptome of the MG during similar proliferative and morphological states. Both estrogen and CLA affected the genes involved in proliferation. The transcriptome for estrogentreated mice included canonical estrogen-induced genes, including Pgr, Areg, and Foxa1. In contrast, their expression was unchanged by CLA. However, CLA, but not estrogen, altered expression of a unique set of inflammation-Associated genes, consistent with stromal changes. This CLA-Altered signature included increased expression of epidermal growth factor receptor (EGFR) pathway components, consistent with the demonstration that CLA-induced MG growth is EGFR dependent. Our findings highlight a unique role for diet-induced inflammation that underlies estrogenindependent MG development. (Endocrinology 158: 3126-3139, 2017).

AB - Allometric growth of ducts in the mammary glands (MGs) is widely held to be estrogen dependent. We previously discovered that the dietary fatty acid trans-10, cis-12 conjugated linoleic acid (CLA) stimulates estrogen-independent allometric growth and terminal end bud formation in ovariectomized mice. Given the similar phenotype induced by estrogen and CLA, we investigated the shared and/or divergent mechanisms underlying these changes. We confirmed MG growth induced by CLA is temporally distinct from that elicited by estrogen. We then used RNA sequencing to compare the transcriptome of the MG during similar proliferative and morphological states. Both estrogen and CLA affected the genes involved in proliferation. The transcriptome for estrogentreated mice included canonical estrogen-induced genes, including Pgr, Areg, and Foxa1. In contrast, their expression was unchanged by CLA. However, CLA, but not estrogen, altered expression of a unique set of inflammation-Associated genes, consistent with stromal changes. This CLA-Altered signature included increased expression of epidermal growth factor receptor (EGFR) pathway components, consistent with the demonstration that CLA-induced MG growth is EGFR dependent. Our findings highlight a unique role for diet-induced inflammation that underlies estrogenindependent MG development. (Endocrinology 158: 3126-3139, 2017).

UR - http://www.scopus.com/inward/record.url?scp=85030635858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030635858&partnerID=8YFLogxK

U2 - 10.1210/en.2017-00395

DO - 10.1210/en.2017-00395

M3 - Article

VL - 158

SP - 3126

EP - 3139

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 10

ER -