The transcription factor basic transcription element-binding protein 1 is a direct thyroid hormone response gene in the frog Xenopus laevis

John Furlow, Akira Kanamori

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Several genes have been identified that are activated or repressed by thyroid hormone in tadpole tissues during metamorphosis of the frog Xenopus laevis. One rapidly and strongly induced gene encodes the Xenopus homolog of basic transcription element-binding protein 1 (xBTEB1), an SP1-related transcription factor. xBTEB1 has similar DNA-binding activity and transcriptional activation properties as mammalian BTEB1. The thyroid hormone-dependent regulation of xBTEB1 was investigated using a modified electrophoretic mobility shift assay to scan genomic DNA for receptor-binding sites. Due to the tetraploid X. laevis genome, xBTEB1 is duplicated, and thyroid hormone regulates both copies. A consensus thyroid hormone response element (TRE) lies far up-stream of the transcriptional start site of both genes. The TRE is nested within a 200-bp region of high sequence conservation between these two genes that duplicated millions of years ago. The TRE acts as a strong response element in transfection assays using a heterologous promoter or its native context. Thus, one of the earliest thyroid hormone-induced genes in tadpoles is a transcription factor regulated through an evolutionarily conserved TRE. xBTEB1 is predicted to play an important role in downstream gene regulation leading to the growth and remodeling of tissues at metamorphosis.

Original languageEnglish (US)
Pages (from-to)3295-3305
Number of pages11
JournalEndocrinology
Volume143
Issue number9
DOIs
StatePublished - Sep 1 2002

Fingerprint

Xenopus laevis
Thyroid Hormones
Anura
Carrier Proteins
Transcription Factors
Response Elements
Genes
Larva
Tetraploidy
Electrophoretic Mobility Shift Assay
Transcriptional Activation
Transfection
Binding Sites
Xenopus BTEB1 protein
Genome
DNA
Growth

ASJC Scopus subject areas

  • Endocrinology

Cite this

The transcription factor basic transcription element-binding protein 1 is a direct thyroid hormone response gene in the frog Xenopus laevis. / Furlow, John; Kanamori, Akira.

In: Endocrinology, Vol. 143, No. 9, 01.09.2002, p. 3295-3305.

Research output: Contribution to journalArticle

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