The TOR-controlled transcription activators GLN3, RTG1, and RTG3 are regulated in response to intracellular levels of glutamine

José L. Crespo, Ted Powers, Brian Fowler, Michael N. Hall

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The essential, rapamycin-sensitive TOR kinases regulate a diverse set of cell growth-related readouts in response to nutrients. Thus, the yeast TOR proteins function as nutrient sensors, in particular as sensors of nitrogen and possibly carbon. However, the nutrient metabolite(s) that acts upstream of TOR is unknown. We investigated the role of glutamine, a preferred nitrogen source and a key intermediate in yeast nitrogen metabolism, as a possible regulator of TOR. We show that the glutamine synthetase inhibitor L-methionine sulfoximine (MSX) specifically provokes glutamine depletion in yeast cells. MSX-induced glutamine starvation caused nuclear localization and activation of the TOR-inhibited transcription factors GLN3, RTG1, and RTG3, all of which mediate glutamine synthesis. The MSX-induced nuclear localization of GLN3 required the TOR-controlled, type 2A-related phosphatase SIT4. Other TOR-controlled transcription factors, GAT1/NIL1, MSN2, MSN4, and an unknown factor involved in the expression of ribosomal protein genes, were not affected by glutamine starvation. These findings suggest that the TOR pathway senses glutamine. Furthermore, as glutamine starvation affects only a subset of TOR-controlled transcription factors, TOR appears to discriminate between different nutrient conditions to elicit a response appropriate to a given condition.

Original languageEnglish (US)
Pages (from-to)6784-6789
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number10
DOIs
StatePublished - May 14 2002

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Glutamine
Methionine Sulfoximine
Starvation
Food
Transcription Factors
Nitrogen
Yeasts
TOR Serine-Threonine Kinases
Glutamate-Ammonia Ligase
Fungal Proteins
Ribosomal Proteins
Sirolimus
Phosphoric Monoester Hydrolases
Methionine
Carbon
Growth
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

The TOR-controlled transcription activators GLN3, RTG1, and RTG3 are regulated in response to intracellular levels of glutamine. / Crespo, José L.; Powers, Ted; Fowler, Brian; Hall, Michael N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 10, 14.05.2002, p. 6784-6789.

Research output: Contribution to journalArticle

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