The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients

Ryan M. Gill, Tzong Hae Lee, Garth H Utter, William F. Reed, Li Wen, Dan Chafets, Michael P. Busch

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n = 30) and without MC (n = 29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

Original languageEnglish (US)
Pages (from-to)3880-3883
Number of pages4
JournalBlood
Volume111
Issue number7
DOIs
StatePublished - Apr 1 2008

Fingerprint

Chimerism
Polymorphism
Nucleotides
Wounds and Injuries
Single Nucleotide Polymorphism
Interleukin-10
Genetic Polymorphisms
Throughput
Modulation
Cytokines
Causality

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Gill, R. M., Lee, T. H., Utter, G. H., Reed, W. F., Wen, L., Chafets, D., & Busch, M. P. (2008). The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients. Blood, 111(7), 3880-3883. https://doi.org/10.1182/blood-2007-08-107144

The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients. / Gill, Ryan M.; Lee, Tzong Hae; Utter, Garth H; Reed, William F.; Wen, Li; Chafets, Dan; Busch, Michael P.

In: Blood, Vol. 111, No. 7, 01.04.2008, p. 3880-3883.

Research output: Contribution to journalArticle

Gill, RM, Lee, TH, Utter, GH, Reed, WF, Wen, L, Chafets, D & Busch, MP 2008, 'The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients', Blood, vol. 111, no. 7, pp. 3880-3883. https://doi.org/10.1182/blood-2007-08-107144
Gill, Ryan M. ; Lee, Tzong Hae ; Utter, Garth H ; Reed, William F. ; Wen, Li ; Chafets, Dan ; Busch, Michael P. / The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients. In: Blood. 2008 ; Vol. 111, No. 7. pp. 3880-3883.
@article{9a4664f812604c96962c4300940b8a33,
title = "The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients",
abstract = "Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n = 30) and without MC (n = 29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.",
author = "Gill, {Ryan M.} and Lee, {Tzong Hae} and Utter, {Garth H} and Reed, {William F.} and Li Wen and Dan Chafets and Busch, {Michael P.}",
year = "2008",
month = "4",
day = "1",
doi = "10.1182/blood-2007-08-107144",
language = "English (US)",
volume = "111",
pages = "3880--3883",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "7",

}

TY - JOUR

T1 - The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients

AU - Gill, Ryan M.

AU - Lee, Tzong Hae

AU - Utter, Garth H

AU - Reed, William F.

AU - Wen, Li

AU - Chafets, Dan

AU - Busch, Michael P.

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n = 30) and without MC (n = 29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

AB - Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n = 30) and without MC (n = 29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

UR - http://www.scopus.com/inward/record.url?scp=43549118444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43549118444&partnerID=8YFLogxK

U2 - 10.1182/blood-2007-08-107144

DO - 10.1182/blood-2007-08-107144

M3 - Article

VL - 111

SP - 3880

EP - 3883

JO - Blood

JF - Blood

SN - 0006-4971

IS - 7

ER -