The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients

Ryan M. Gill, Tzong Hae Lee, Garth H Utter, William F. Reed, Li Wen, Dan Chafets, Michael P. Busch

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n = 30) and without MC (n = 29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

Original languageEnglish (US)
Pages (from-to)3880-3883
Number of pages4
JournalBlood
Volume111
Issue number7
DOIs
StatePublished - Apr 1 2008

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Fingerprint Dive into the research topics of 'The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients'. Together they form a unique fingerprint.

  • Cite this

    Gill, R. M., Lee, T. H., Utter, G. H., Reed, W. F., Wen, L., Chafets, D., & Busch, M. P. (2008). The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients. Blood, 111(7), 3880-3883. https://doi.org/10.1182/blood-2007-08-107144