The thuEFGKAB operon of Rhizobia and Agrobacterium tumefaciens codes for transport of trehalose, maltitol, and isomers of sucrose and their assimilation through the formation of their 3-keto derivatives

Osei Yaw Ampomah, Anna Avetisyan, Espen Hansen, Johan Svenson, Thomas R Huser, John Beck Jensen, T. V. Bhuvaneswari

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The thu operon (thuEFGKAB) in Sinorhizobium meliloti codes for transport and utilization functions of the disaccharide trehalose. Sequenced genomes of members of the Rhizobiaceae reveal that some rhizobia and Agrobacterium possess the entire thu operon in similar organizations and that Mesorhizobium loti MAFF303099 lacks the transport (thuEFGK) genes. In this study, we show that this operon is dedicated to the transport and assimilation of maltitol and isomers of sucrose (leucrose, palatinose, and trehalulose) in addition to trehalulose, not only in S. meliloti but also in Agrobacterium tumefaciens. By using genetic complementation, we show that the thuAB genes of S. meliloti, M. loti, and A. tumefaciens are functionally equivalent. Further, we provide both genetic and biochemical evidence to show that these bacteria assimilate these disaccharides by converting them to their respective 3-keto derivatives and that the thuAB genes code for this ketodisaccharide-forming enzyme(s). Formation of 3-ketotrehalose in real time in live S. meliloti is shown through Raman spectroscopy. The presence of an additional ketodisaccharide- forming pathway(s) in A. tumefaciens is also indicated. To our knowledge, this is the first report to identify the genes that code for the conversion of disaccharides to their 3-ketodisaccharide derivatives in any organism.

Original languageEnglish (US)
Pages (from-to)3797-3807
Number of pages11
JournalJournal of Bacteriology
Volume195
Issue number17
DOIs
StatePublished - 2013

Fingerprint

Sinorhizobium meliloti
Agrobacterium tumefaciens
Trehalose
Rhizobium
Operon
Sucrose
Disaccharides
Rhizobiaceae
Mesorhizobium
Genes
Gene Conversion
Agrobacterium
Raman Spectrum Analysis
Molecular Biology
Genome
Bacteria
maltitol
Enzymes
trehalulose

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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The thuEFGKAB operon of Rhizobia and Agrobacterium tumefaciens codes for transport of trehalose, maltitol, and isomers of sucrose and their assimilation through the formation of their 3-keto derivatives. / Ampomah, Osei Yaw; Avetisyan, Anna; Hansen, Espen; Svenson, Johan; Huser, Thomas R; Jensen, John Beck; Bhuvaneswari, T. V.

In: Journal of Bacteriology, Vol. 195, No. 17, 2013, p. 3797-3807.

Research output: Contribution to journalArticle

Ampomah, Osei Yaw ; Avetisyan, Anna ; Hansen, Espen ; Svenson, Johan ; Huser, Thomas R ; Jensen, John Beck ; Bhuvaneswari, T. V. / The thuEFGKAB operon of Rhizobia and Agrobacterium tumefaciens codes for transport of trehalose, maltitol, and isomers of sucrose and their assimilation through the formation of their 3-keto derivatives. In: Journal of Bacteriology. 2013 ; Vol. 195, No. 17. pp. 3797-3807.
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