The temporal sequence of spontaneous repair of osteochondral defects in the knees of rabbits is dependent on the geometry of the defect

S. A. Lietman, S. Miyamoto, P. R. Brown, N. Inoue, A Hari Reddi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Damage to articular cartilage is a common injury, for which there is no effective treatment. Our aims were to investigate the temporal sequence of the repair of articular cartilage and to define a critical-size defect. Full-thickness defects were made in adult male New Zealand white rabbits. The diameter (1 to 4 mm) of the defects was varied in order to determine the effect that the size and depth of the defect had on its healing. The defects were made in the femoral groove of the knee with one defect per knee and eight knees per group. The tissues were fixed in formalin at days 3, 7, 14, 21, 28, 42, 84 and 126 after operation and the sections stained with Toluidine Blue. These were then examined and evaluated for several parameters including the degree of metachromasia and the amount of subchondral bone which had reformed in the defect. The defects had a characteristic pattern of healing which differed at different days and for different sizes of defect. Specifically, the defects of 1 mm first peaked in terms of metachromasia at day 21, those of 2 mm at day 28, followed by defects of 3 mm and 4 mm. The healing of the subchondral bone was slowest in defects of 1 mm.

Original languageEnglish (US)
Pages (from-to)600-606
Number of pages7
JournalJournal of Bone and Joint Surgery - Series B
Volume84
Issue number4
StatePublished - 2002
Externally publishedYes

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Knee
Articular Cartilage
Rabbits
Bone and Bones
Tolonium Chloride
Thigh
Formaldehyde
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

The temporal sequence of spontaneous repair of osteochondral defects in the knees of rabbits is dependent on the geometry of the defect. / Lietman, S. A.; Miyamoto, S.; Brown, P. R.; Inoue, N.; Reddi, A Hari.

In: Journal of Bone and Joint Surgery - Series B, Vol. 84, No. 4, 2002, p. 600-606.

Research output: Contribution to journalArticle

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