The subventricular zone continues to generate corpus callosum and rostral migratory stream astroglia in normal adult mice

Jiho Sohn, Lori Orosco, Fuzheng Guo, Seung Hyuk Chung, Peter Bannerman, Emily Mills Ko, Konstantinos Zarbalis, Wenbin Deng, David E Pleasure

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Astrocytes are the most abundant cells in the CNS, and have many essential functions, including maintenance of blood- brain barrier integrity, and CNS water, ion, and glutamate homeostasis. Mammalian astrogliogenesis has generally been considered to be completed soon after birth, and to be reactivated in later life only under pathological circumstances. Here, by using genetic fate-mapping, we demonstrate that new corpus callosum astrocytes are continuously generated from nestin+ subventricular zone (SVZ) neural progenitor cells (NPCs) in normal adult mice. These nestin fate-mapped corpus callosum astrocytes are uniformly postmitotic, express glutamate receptors, and form aquaporin-4+ perivascular endfeet. The entry of new astrocytes from the SVZ into the corpus callosum appears to be balanced by astroglial apoptosis, because overall numbers of corpus callosum astrocytes remain constant during normal adulthood. Nestin fate-mapped astrocytes also flow anteriorly from the SVZ in association with the rostral migratory stream, but do not penetrate into the deeper layers of the olfactory bulb. Production of new astrocytes from nestin+ NPCs is absent in the normal adult cortex, striatum, and spinal cord. Our study is the first to demonstrate ongoing SVZ astrogliogenesis in the normal adult mammalian forebrain.

Original languageEnglish (US)
Pages (from-to)3756-3763
Number of pages8
JournalJournal of Neuroscience
Volume35
Issue number9
DOIs
StatePublished - 2015

Fingerprint

Corpus Callosum
Lateral Ventricles
Astrocytes
Nestin
Stem Cells
Aquaporin 4
Olfactory Bulb
Glutamate Receptors
Prosencephalon
Blood-Brain Barrier
Glutamic Acid
Spinal Cord
Homeostasis
Maintenance
Parturition
Ions
Apoptosis
Water

Keywords

  • Astroglia
  • Corpus callosum
  • Genetic fate-mapping
  • Neural progenitor cells
  • Rostral migratory stream
  • Subventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The subventricular zone continues to generate corpus callosum and rostral migratory stream astroglia in normal adult mice. / Sohn, Jiho; Orosco, Lori; Guo, Fuzheng; Chung, Seung Hyuk; Bannerman, Peter; Ko, Emily Mills; Zarbalis, Konstantinos; Deng, Wenbin; Pleasure, David E.

In: Journal of Neuroscience, Vol. 35, No. 9, 2015, p. 3756-3763.

Research output: Contribution to journalArticle

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