The structure of vimentin linker 1 and rod 1B domains characterized by site-directed spin-labeling electron paramagnetic resonance (SDSL-EPR) and X-ray crystallography

Atya Aziz, John F. Hess, Madhu S. Budamagunta, John C Voss, Alexandre P. Kuzin, Yuanpeng J. Huang, Rong Xiao, Gaetano T. Montelione, Paul G FitzGerald, John F. Hunt

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Despite the passage of ∼30 years since the complete primary sequence of the intermediate filament (IF) protein vimentin was reported, the structure remains unknown for both an individual protomer and the assembled filament. In this report, we present data describing the structure of vimentin linker 1 (L1) and rod 1B. Electron paramagnetic resonance spectra collected from samples bearing site-directed spin labels demonstrate that L1 is not a flexible segment between coiled-coils (CCs) but instead forms a rigid, tightly packed structure. Anx-ray crystal structure of a construct containing L1 and rod 1B shows that it forms a tetramer comprising two equivalent parallel CC dimers that interact with one another in the form of a symmetrical antiparallel dimer. Remarkably, the parallel CC dimers are themselves asymmetrical, which enables them to tetramerize rather than undergoing higher order oligomerization. This functionally vital asymmetry in theCCstructure, encoded in the primary sequence of rod 1B, provides a striking example of evolutionary exploitation of the structural plasticity of proteins. EPR and crystallographic data consistently suggest that a very short region within L1 represents a minor local distortion in what is likely to be a continuous CC from the end of rod 1A through the entirety of rod 1B. The concordance of this structural model with previously published cross-linking and spectral data supports the conclusion that the crystallographic oligomer represents a native biological structure.

Original languageEnglish (US)
Pages (from-to)28349-28361
Number of pages13
JournalJournal of Biological Chemistry
Volume287
Issue number34
DOIs
StatePublished - Aug 17 2012

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X ray crystallography
X Ray Crystallography
Electron Spin Resonance Spectroscopy
Vimentin
Dimers
Labeling
Paramagnetic resonance
Intermediate Filament Proteins
Spin Labels
Structural Models
Protein Subunits
Bearings (structural)
Oligomerization
Oligomers
Plasticity
Crystal structure
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The structure of vimentin linker 1 and rod 1B domains characterized by site-directed spin-labeling electron paramagnetic resonance (SDSL-EPR) and X-ray crystallography. / Aziz, Atya; Hess, John F.; Budamagunta, Madhu S.; Voss, John C; Kuzin, Alexandre P.; Huang, Yuanpeng J.; Xiao, Rong; Montelione, Gaetano T.; FitzGerald, Paul G; Hunt, John F.

In: Journal of Biological Chemistry, Vol. 287, No. 34, 17.08.2012, p. 28349-28361.

Research output: Contribution to journalArticle

Aziz, Atya ; Hess, John F. ; Budamagunta, Madhu S. ; Voss, John C ; Kuzin, Alexandre P. ; Huang, Yuanpeng J. ; Xiao, Rong ; Montelione, Gaetano T. ; FitzGerald, Paul G ; Hunt, John F. / The structure of vimentin linker 1 and rod 1B domains characterized by site-directed spin-labeling electron paramagnetic resonance (SDSL-EPR) and X-ray crystallography. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 34. pp. 28349-28361.
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