Abstract
A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.
Original language | English (US) |
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Pages (from-to) | 99-108 |
Number of pages | 10 |
Journal | Experimental Cell Research |
Volume | 105 |
Issue number | 1 |
DOIs | |
State | Published - Mar 1 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology