The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

M. Klagsbrun; R. Langer; Richard M Levenson; S. Smith; C. Lillehei

doi:10.1016/0014-4827(77)90155-0

The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

M. Klagsbrun, R. Langer, Richard M Levenson, S. Smith, C. Lillehei

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.

Original language	English (US)
Pages (from-to)	99-108
Number of pages	10
Journal	Experimental Cell Research
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(77)90155-0
State	Published - Mar 1 1977
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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AU - Klagsbrun, M.

AU - Langer, R.

AU - Levenson, Richard M

AU - Smith, S.

AU - Lillehei, C.

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AB - A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.

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