The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

M. Klagsbrun; R. Langer; Richard M Levenson; S. Smith; C. Lillehei

doi:10.1016/0014-4827(77)90155-0

The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

M. Klagsbrun, R. Langer, Richard M Levenson, S. Smith, C. Lillehei

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.

Original language	English (US)
Pages (from-to)	99-108
Number of pages	10
Journal	Experimental Cell Research
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(77)90155-0
State	Published - Mar 1 1977
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0014-4827(77)90155-0

Fingerprint Dive into the research topics of 'The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage'. Together they form a unique fingerprint.

Cite this

@article{12af65a4490e4c0b942c9af9425fcb65,

title = "The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage",

abstract = "A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.",

author = "M. Klagsbrun and R. Langer and Levenson, {Richard M} and S. Smith and C. Lillehei",

year = "1977",

month = mar,

day = "1",

doi = "10.1016/0014-4827(77)90155-0",

language = "English (US)",

volume = "105",

pages = "99--108",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

AU - Klagsbrun, M.

AU - Langer, R.

AU - Levenson, Richard M

AU - Smith, S.

AU - Lillehei, C.

PY - 1977/3/1

Y1 - 1977/3/1

N2 - A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.

AB - A growth factor which stimulates DNA synthesis and cell division in resting monolayers of bovine chondrocytes and confluent BALB/c 3T3 cells has been extracted from bovine scapular cartilage and partially purified. Chromatography by gel filtration in the presence of 4 M guanidine hydrochloride indicates that the growth factor has a molecular weight of approx. 11000 to 13000 D. Ion exchange chromatography indicates that the growth factor is cationic. The biological activity of the cartilage-derived growth factor is destroyed by trypsin digestion but not by incubation with 2-mercaptoethanol. The stimulation of cell division in BALB/c 3T3 cells is accompanied by changes in cell morphology.

UR - http://www.scopus.com/inward/record.url?scp=0017340895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017340895&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(77)90155-0

DO - 10.1016/0014-4827(77)90155-0

M3 - Article

C2 - 556995

AN - SCOPUS:0017340895

VL - 105

SP - 99

EP - 108

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -

The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this