Abstract
Homologous recombination is a ubiquitous process with key functions in meiotic and vegetative cells for the repair of DNA breaks. It is initiated by the formation of single-stranded DNA on which recombination proteins bind to form a nucleoprotein filament that is active in searching for homology, in the formation of joint molecules and in the exchange of DNA strands1. This process contributes to genome stability but it is also potentially dangerous to cells if intermediates are formed that cannot be processed normally and thus are toxic or generate genomic rearrangements. Cells must therefore have developed strategies to survey recombination and to prevent the occurrence of such deleterious events. In Saccharomyces cerevisiae, genetic data have shown that the Srs2 helicase negatively modulates recombination2,3, and later experiments suggested that it reverses intermediate recombination structures4-7. Here we show that DNA strand exchange mediated in vitro by Rad51 is inhibited by Srs2, and that Srs2 disrupts Rad51 filaments formed on single-stranded DNA. These data provide an explanation for the anti-recombinogenic role of Srs2 in vivo and highlight a previously unknown mechanism for recombination control.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 309-312 |
| Number of pages | 4 |
| Journal | Nature |
| Volume | 423 |
| Issue number | 6937 |
| DOIs | |
| State | Published - May 15 2003 |
Fingerprint
ASJC Scopus subject areas
- General
Cite this
The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments. / Veaute, Xavier; Jeusset, Josette; Soustelle, Christine; Kowalczykowski, Stephen C.; Le Cam, Eric; Fahre, Francis.
In: Nature, Vol. 423, No. 6937, 15.05.2003, p. 309-312.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments
AU - Veaute, Xavier
AU - Jeusset, Josette
AU - Soustelle, Christine
AU - Kowalczykowski, Stephen C.
AU - Le Cam, Eric
AU - Fahre, Francis
PY - 2003/5/15
Y1 - 2003/5/15
N2 - Homologous recombination is a ubiquitous process with key functions in meiotic and vegetative cells for the repair of DNA breaks. It is initiated by the formation of single-stranded DNA on which recombination proteins bind to form a nucleoprotein filament that is active in searching for homology, in the formation of joint molecules and in the exchange of DNA strands1. This process contributes to genome stability but it is also potentially dangerous to cells if intermediates are formed that cannot be processed normally and thus are toxic or generate genomic rearrangements. Cells must therefore have developed strategies to survey recombination and to prevent the occurrence of such deleterious events. In Saccharomyces cerevisiae, genetic data have shown that the Srs2 helicase negatively modulates recombination2,3, and later experiments suggested that it reverses intermediate recombination structures4-7. Here we show that DNA strand exchange mediated in vitro by Rad51 is inhibited by Srs2, and that Srs2 disrupts Rad51 filaments formed on single-stranded DNA. These data provide an explanation for the anti-recombinogenic role of Srs2 in vivo and highlight a previously unknown mechanism for recombination control.
AB - Homologous recombination is a ubiquitous process with key functions in meiotic and vegetative cells for the repair of DNA breaks. It is initiated by the formation of single-stranded DNA on which recombination proteins bind to form a nucleoprotein filament that is active in searching for homology, in the formation of joint molecules and in the exchange of DNA strands1. This process contributes to genome stability but it is also potentially dangerous to cells if intermediates are formed that cannot be processed normally and thus are toxic or generate genomic rearrangements. Cells must therefore have developed strategies to survey recombination and to prevent the occurrence of such deleterious events. In Saccharomyces cerevisiae, genetic data have shown that the Srs2 helicase negatively modulates recombination2,3, and later experiments suggested that it reverses intermediate recombination structures4-7. Here we show that DNA strand exchange mediated in vitro by Rad51 is inhibited by Srs2, and that Srs2 disrupts Rad51 filaments formed on single-stranded DNA. These data provide an explanation for the anti-recombinogenic role of Srs2 in vivo and highlight a previously unknown mechanism for recombination control.
UR - http://www.scopus.com/inward/record.url?scp=0037673943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037673943&partnerID=8YFLogxK
U2 - 10.1038/nature01585
DO - 10.1038/nature01585
M3 - Article
C2 - 12748645
AN - SCOPUS:0037673943
VL - 423
SP - 309
EP - 312
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6937
ER -