The spindle checkpoint of Saccharomyces cerevisiae responds to separable microtubule-dependent events

John R. Daum, Natalia Gomez-Ospina, Mark Winey, Daniel J. Burke

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The spindle checkpoint regulates microtubule-based chromosome segregation and helps to maintain genomic stability [1,2]. Mutational inactivation of spindle checkpoint genes has been implicated in the progression of several types of human cancer. Recent evidence from budding yeast suggests that the spindle checkpoint is complex. Order-of-function experiments have defined two separable pathways within the checkpoint. One pathway, defined by MAD2, controls the metaphase-to-anaphase transition and the other, defined by BUB2, controls the exit from mitosis [3-6]. The relationships between the separate branches of the checkpoint, and especially the events that trigger the pathways, have not been defined. We localized a Bub2p-GFP fusion protein to the cytoplasmic side of the spindle pole body and used a kar9 mutant to show that cells with misoriented spindles are arrested in anaphase of mitosis. We used a kar9 bub2 double mutant to show that the arrest is BUB2 dependent. We conclude that the separate pathways of the spindle checkpoint respond to different classes of microtubules. The MAD2 branch of the pathway responds to kinetochore microtubule interactions and the BUB2 branch of the pathway operates within the cytoplasm, responding to spindle misorientation.

Original languageEnglish (US)
Pages (from-to)1375-1378
Number of pages4
JournalCurrent Biology
Volume10
Issue number21
DOIs
StatePublished - Nov 2 2000
Externally publishedYes

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Microtubules
Yeast
microtubules
Saccharomyces cerevisiae
Anaphase
anaphase
Mitosis
mitosis
Chromosomes
Spindle Pole Bodies
spindle pole body
Kinetochores
Poles
kinetochores
mutants
Chromosome Segregation
Saccharomycetales
Fusion reactions
chromosome segregation
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The spindle checkpoint of Saccharomyces cerevisiae responds to separable microtubule-dependent events. / Daum, John R.; Gomez-Ospina, Natalia; Winey, Mark; Burke, Daniel J.

In: Current Biology, Vol. 10, No. 21, 02.11.2000, p. 1375-1378.

Research output: Contribution to journalArticle

Daum, John R. ; Gomez-Ospina, Natalia ; Winey, Mark ; Burke, Daniel J. / The spindle checkpoint of Saccharomyces cerevisiae responds to separable microtubule-dependent events. In: Current Biology. 2000 ; Vol. 10, No. 21. pp. 1375-1378.
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