The spectrin family member Syne-1 functions in retrograde transport from Golgi to ER

Lisa Lucio Gough, Kenneth A Beck

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To address the function of the Golgi- and nuclear envelope-localized spectrin family member synaptic nuclear envelope protein-1 (Syne-1), we expressed two separate recombinant fragments derived from the central portion of the molecule. Both of these fragments were predicted to act as dominant negative inhibitors of Syne-1 function at the Golgi. One of the fragments was previously shown to bind the Golgi complex. The other fragment was found to form microtubule-associated puncta that sequester endogenous Syne-1. Expression of either fragment resulted in a cell type-specific alteration in the structure of the Golgi complex, which appeared to collapse into a compact juxtanuclear structure in some cell types but not others. These fragments were expressed in cultured cells and their effects on Golgi function were examined. Expression of both dominant negative Syne-1 fragments blocked recycling of the endoplasmic reticulum (ER) resident protein disulfide isomerase (PDI), which accumulated in the Golgi complex. In addition, we found that fragment expression altered the distribution of the KDEL receptor and the COP-I coat protein β-COP, two proteins known to be involved in regulating the retrograde pathway. We conclude that these results indicate a role for Syne-1 in facilitating retrograde vesicular trafficking from the Golgi to the ER.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1693
Issue number1
DOIs
StatePublished - Jul 23 2004

Fingerprint

Spectrin
Nuclear Envelope
Nuclear Proteins
Endoplasmic Reticulum
Golgi Apparatus
Coat Protein Complex I
Protein Disulfide-Isomerases
Capsid Proteins
Microtubules
Cultured Cells
Proteins

Keywords

  • bovine serum albumin
  • BSA
  • endoplasmic reticulum
  • Endoplasmic reticulum
  • ER
  • Golgi
  • Madin-Darby canine kidney
  • MDCK
  • Microtubule
  • PBS
  • PDI
  • phosphate-buffered saline
  • Retrograde vesicle transport
  • Spectrin
  • synaptic nuclear envelope protein-1
  • Syne-1

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

The spectrin family member Syne-1 functions in retrograde transport from Golgi to ER. / Gough, Lisa Lucio; Beck, Kenneth A.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1693, No. 1, 23.07.2004, p. 29-36.

Research output: Contribution to journalArticle

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