The specific inhibition of cellular clonogenic proliferation using 211At labelled lectins and antibodies-I

Andrew T M Vaughan; W. J. Bateman; G. Brown; J. Cowan

doi:10.1016/0047-0740(82)90091-2

The specific inhibition of cellular clonogenic proliferation using ²¹¹At labelled lectins and antibodies-I

Andrew T M Vaughan, W. J. Bateman, G. Brown, J. Cowan

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A monoclonal antibody with a broad anti-human leucocyte specificity, designated BK 19.45 and the plant lectin Concanavalin A have been labelled with the α-emitting cyclotron produced radiohalogen astatine-211. Both human and murine tumour cell lines and human leukemic bone marrow samples have been specifically labelled with these radioactive proteins. In all cases the amount of ²¹¹At bound to the cells is directly correlated with a decrease in cellular reproductive potential as shown by the ability of the cells to proliferate in a clonogenic assay. For the labelled monoclonal antibody experiments, the radiation dose needed to yield 37% cell survival, the D₃₇ dose, may be achieved with an averaged of 12²¹¹ At atoms/cell.

Original language	English (US)
Pages (from-to)	167-171
Number of pages	5
Journal	International Journal of Nuclear Medicine and Biology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/0047-0740(82)90091-2
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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title = "The specific inhibition of cellular clonogenic proliferation using 211At labelled lectins and antibodies-I",

abstract = "A monoclonal antibody with a broad anti-human leucocyte specificity, designated BK 19.45 and the plant lectin Concanavalin A have been labelled with the α-emitting cyclotron produced radiohalogen astatine-211. Both human and murine tumour cell lines and human leukemic bone marrow samples have been specifically labelled with these radioactive proteins. In all cases the amount of 211At bound to the cells is directly correlated with a decrease in cellular reproductive potential as shown by the ability of the cells to proliferate in a clonogenic assay. For the labelled monoclonal antibody experiments, the radiation dose needed to yield 37% cell survival, the D37 dose, may be achieved with an averaged of 12211 At atoms/cell.",

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AU - Vaughan, Andrew T M

AU - Bateman, W. J.

AU - Brown, G.

AU - Cowan, J.

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N2 - A monoclonal antibody with a broad anti-human leucocyte specificity, designated BK 19.45 and the plant lectin Concanavalin A have been labelled with the α-emitting cyclotron produced radiohalogen astatine-211. Both human and murine tumour cell lines and human leukemic bone marrow samples have been specifically labelled with these radioactive proteins. In all cases the amount of 211At bound to the cells is directly correlated with a decrease in cellular reproductive potential as shown by the ability of the cells to proliferate in a clonogenic assay. For the labelled monoclonal antibody experiments, the radiation dose needed to yield 37% cell survival, the D37 dose, may be achieved with an averaged of 12211 At atoms/cell.

AB - A monoclonal antibody with a broad anti-human leucocyte specificity, designated BK 19.45 and the plant lectin Concanavalin A have been labelled with the α-emitting cyclotron produced radiohalogen astatine-211. Both human and murine tumour cell lines and human leukemic bone marrow samples have been specifically labelled with these radioactive proteins. In all cases the amount of 211At bound to the cells is directly correlated with a decrease in cellular reproductive potential as shown by the ability of the cells to proliferate in a clonogenic assay. For the labelled monoclonal antibody experiments, the radiation dose needed to yield 37% cell survival, the D37 dose, may be achieved with an averaged of 12211 At atoms/cell.

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