The specific inhibition of cellular clonogenic proliferation using 211At labelled lectins and antibodies-I

Andrew T M Vaughan; W. J. Bateman; G. Brown; J. Cowan

doi:10.1016/0047-0740(82)90091-2

The specific inhibition of cellular clonogenic proliferation using ²¹¹At labelled lectins and antibodies-I

Andrew T M Vaughan, W. J. Bateman, G. Brown, J. Cowan

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Abstract

A monoclonal antibody with a broad anti-human leucocyte specificity, designated BK 19.45 and the plant lectin Concanavalin A have been labelled with the α-emitting cyclotron produced radiohalogen astatine-211. Both human and murine tumour cell lines and human leukemic bone marrow samples have been specifically labelled with these radioactive proteins. In all cases the amount of ²¹¹At bound to the cells is directly correlated with a decrease in cellular reproductive potential as shown by the ability of the cells to proliferate in a clonogenic assay. For the labelled monoclonal antibody experiments, the radiation dose needed to yield 37% cell survival, the D₃₇ dose, may be achieved with an averaged of 12²¹¹ At atoms/cell.

Original language	English (US)
Pages (from-to)	167-171
Number of pages	5
Journal	International Journal of Nuclear Medicine and Biology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/0047-0740(82)90091-2
State	Published - 1982
Externally published	Yes

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ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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Vaughan, A. T. M., Bateman, W. J., Brown, G., & Cowan, J. (1982). The specific inhibition of cellular clonogenic proliferation using ²¹¹At labelled lectins and antibodies-I. International Journal of Nuclear Medicine and Biology, 9(3), 167-171. https://doi.org/10.1016/0047-0740(82)90091-2

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10.1016/0047-0740(82)90091-2