The SMRT corepressor is a target of phosphorylation by protein kinase CK2 (casein kinase II)

Y. Zhou, W. Gross, S. H. Hong, M. L. Privalsky

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The Silencing-Mediator for Retinoid/Thyroid hormone receptors (SMRT) interacts with, and mediates transcriptional repression by, a variety of eukaryotic transcription factors, including the nuclear hormone receptors. The ability of SMRT to function as a transcriptional 'corepressor' is regulated by a variety of signal transduction pathways. We report here that SMRT is a phosphoprotein in vivo, and is also phosphorylated in vitro by unfractionated cell extracts. A major site of phosphorylation of SMRT is a protein kinase CK2 motif centered on serine 1492, and located within a C-terminal SMRT domain that mediates interaction of the corepressor with the nuclear hormone receptors. Phosphorylation of SMRT by CK2 stabilizes the ability of the SMRT protein to interact with the nuclear hormone receptors. Our results indicate that SMRT is a member of an expanding family of transcriptional regulators that are modified, and potentially regulated, in response to protein kinase CK2.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalMolecular and Cellular Biochemistry
Volume220
Issue number1-2
DOIs
StatePublished - 2001

Fingerprint

Casein Kinase II
Thyroid Hormone Receptors
Co-Repressor Proteins
Phosphorylation
Retinoids
Cytoplasmic and Nuclear Receptors
Aptitude
Signal transduction
Phosphoproteins
Cell Extracts
Serine
Signal Transduction
Transcription Factors

Keywords

  • Casein kinase 2
  • CK2
  • Corepressor
  • Nuclear hormone receptors
  • Protein kinase

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The SMRT corepressor is a target of phosphorylation by protein kinase CK2 (casein kinase II). / Zhou, Y.; Gross, W.; Hong, S. H.; Privalsky, M. L.

In: Molecular and Cellular Biochemistry, Vol. 220, No. 1-2, 2001, p. 1-13.

Research output: Contribution to journalArticle

Zhou, Y. ; Gross, W. ; Hong, S. H. ; Privalsky, M. L. / The SMRT corepressor is a target of phosphorylation by protein kinase CK2 (casein kinase II). In: Molecular and Cellular Biochemistry. 2001 ; Vol. 220, No. 1-2. pp. 1-13.
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