Abstract
The silver (svr) gene of Drosophila melanogaster is required for viability, and severe mutant alleles result in death prior to eclosion. Adult flies homozygous or hemizygous for weaker alleles display several visible phenotypes, including cuticular structures that are pale and silvery in color due to reduced melanization. We have identified and cloned the DNA encoding the svr gene and determined the sequence of several partially overlapping cDNAs derived from svr mRNAs. The predicted amino acid sequence of the polypeptides encoded by these cDNAs indicates thai the silver proteins are members of the family of preprotein-processing carboxypeptidases that includes the human carboxypeptidases E, M, and N. One class of svr mRNAs is alternatively spliced to encode at least two polyproteins, each of which is composed of two carboxypeptidase domains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9470-9474 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 92 |
| Issue number | 21 |
| DOIs | |
| State | Published - Oct 10 1995 |
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Keywords
- melanization
- neuropeptide
- polyprotein
- svr gene
ASJC Scopus subject areas
- Genetics
- General
Cite this
The silver gene of Drosophila melanogaster encodes multiple carboxypeptidases similar to mammalian prohormone-processing enzymes. / Settle, Stephen H.; Green, M. M.; Burtis, Kenneth C.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 21, 10.10.1995, p. 9470-9474.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The silver gene of Drosophila melanogaster encodes multiple carboxypeptidases similar to mammalian prohormone-processing enzymes
AU - Settle, Stephen H.
AU - Green, M. M.
AU - Burtis, Kenneth C.
PY - 1995/10/10
Y1 - 1995/10/10
N2 - The silver (svr) gene of Drosophila melanogaster is required for viability, and severe mutant alleles result in death prior to eclosion. Adult flies homozygous or hemizygous for weaker alleles display several visible phenotypes, including cuticular structures that are pale and silvery in color due to reduced melanization. We have identified and cloned the DNA encoding the svr gene and determined the sequence of several partially overlapping cDNAs derived from svr mRNAs. The predicted amino acid sequence of the polypeptides encoded by these cDNAs indicates thai the silver proteins are members of the family of preprotein-processing carboxypeptidases that includes the human carboxypeptidases E, M, and N. One class of svr mRNAs is alternatively spliced to encode at least two polyproteins, each of which is composed of two carboxypeptidase domains.
AB - The silver (svr) gene of Drosophila melanogaster is required for viability, and severe mutant alleles result in death prior to eclosion. Adult flies homozygous or hemizygous for weaker alleles display several visible phenotypes, including cuticular structures that are pale and silvery in color due to reduced melanization. We have identified and cloned the DNA encoding the svr gene and determined the sequence of several partially overlapping cDNAs derived from svr mRNAs. The predicted amino acid sequence of the polypeptides encoded by these cDNAs indicates thai the silver proteins are members of the family of preprotein-processing carboxypeptidases that includes the human carboxypeptidases E, M, and N. One class of svr mRNAs is alternatively spliced to encode at least two polyproteins, each of which is composed of two carboxypeptidase domains.
KW - melanization
KW - neuropeptide
KW - polyprotein
KW - svr gene
UR - http://www.scopus.com/inward/record.url?scp=0028882212&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028882212&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.21.9470
DO - 10.1073/pnas.92.21.9470
M3 - Article
C2 - 7568156
AN - SCOPUS:0028882212
VL - 92
SP - 9470
EP - 9474
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 21
ER -