Abstract
Little is known about factors which enable Salmonella serotypes to circulate within populations of livestock and domestic fowl. We have identified a DNA region which is present in Salmonella serotypes commonly isolated from livestock and domestic fowl (S. enterica subspecies I) but absent from reptile-associated Salmonella serotypes (S. bongori and S. enterica subspecies II to VII). This DNA region was cloned from Salmonella serotype Typhimurium and sequence analysis revealed the presence of a 6,105- bp open reading frame, designated shdA, whose product's deduced amino acid sequence displayed homology to that of AIDA-I from diarrheagenic Escherichia coli, MisL of serotype Typhimurium, and IcsA of Shigella flexneri. The shdA gene was located adjacent to xseA at 52 min, in a 30-kb DNA region which is not present in Escherichia coli K-12. A serotype Typhimurium shdA mutant was shed with the feces in reduced numbers and for a shorter period of time compared to its isogenic parent. A possible role for the shdA gene during the expansion in host range of S. enterica subspecies I to include warm-blooded vertebrates is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2720-2727 |
| Number of pages | 8 |
| Journal | Infection and Immunity |
| Volume | 68 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
The shdA gene is restricted to serotypes of Salmonella enterica subspecies I and contributes to efficient and prolonged fecal shedding. / Kingsley, Robert A.; Van Amsterdam, Karin; Kramer, Naomi; Baumler, Andreas J.
In: Infection and Immunity, Vol. 68, No. 5, 05.2000, p. 2720-2727.Research output: Contribution to journal › Article
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TY - JOUR
T1 - The shdA gene is restricted to serotypes of Salmonella enterica subspecies I and contributes to efficient and prolonged fecal shedding
AU - Kingsley, Robert A.
AU - Van Amsterdam, Karin
AU - Kramer, Naomi
AU - Baumler, Andreas J
PY - 2000/5
Y1 - 2000/5
N2 - Little is known about factors which enable Salmonella serotypes to circulate within populations of livestock and domestic fowl. We have identified a DNA region which is present in Salmonella serotypes commonly isolated from livestock and domestic fowl (S. enterica subspecies I) but absent from reptile-associated Salmonella serotypes (S. bongori and S. enterica subspecies II to VII). This DNA region was cloned from Salmonella serotype Typhimurium and sequence analysis revealed the presence of a 6,105- bp open reading frame, designated shdA, whose product's deduced amino acid sequence displayed homology to that of AIDA-I from diarrheagenic Escherichia coli, MisL of serotype Typhimurium, and IcsA of Shigella flexneri. The shdA gene was located adjacent to xseA at 52 min, in a 30-kb DNA region which is not present in Escherichia coli K-12. A serotype Typhimurium shdA mutant was shed with the feces in reduced numbers and for a shorter period of time compared to its isogenic parent. A possible role for the shdA gene during the expansion in host range of S. enterica subspecies I to include warm-blooded vertebrates is discussed.
AB - Little is known about factors which enable Salmonella serotypes to circulate within populations of livestock and domestic fowl. We have identified a DNA region which is present in Salmonella serotypes commonly isolated from livestock and domestic fowl (S. enterica subspecies I) but absent from reptile-associated Salmonella serotypes (S. bongori and S. enterica subspecies II to VII). This DNA region was cloned from Salmonella serotype Typhimurium and sequence analysis revealed the presence of a 6,105- bp open reading frame, designated shdA, whose product's deduced amino acid sequence displayed homology to that of AIDA-I from diarrheagenic Escherichia coli, MisL of serotype Typhimurium, and IcsA of Shigella flexneri. The shdA gene was located adjacent to xseA at 52 min, in a 30-kb DNA region which is not present in Escherichia coli K-12. A serotype Typhimurium shdA mutant was shed with the feces in reduced numbers and for a shorter period of time compared to its isogenic parent. A possible role for the shdA gene during the expansion in host range of S. enterica subspecies I to include warm-blooded vertebrates is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0034114755&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034114755&partnerID=8YFLogxK
U2 - 10.1128/IAI.68.5.2720-2727.2000
DO - 10.1128/IAI.68.5.2720-2727.2000
M3 - Article
C2 - 10768965
AN - SCOPUS:0034114755
VL - 68
SP - 2720
EP - 2727
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 5
ER -