The shdA gene is restricted to serotypes of Salmonella enterica subspecies I and contributes to efficient and prolonged fecal shedding

Robert A. Kingsley, Karin Van Amsterdam, Naomi Kramer, Andreas J Baumler

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Little is known about factors which enable Salmonella serotypes to circulate within populations of livestock and domestic fowl. We have identified a DNA region which is present in Salmonella serotypes commonly isolated from livestock and domestic fowl (S. enterica subspecies I) but absent from reptile-associated Salmonella serotypes (S. bongori and S. enterica subspecies II to VII). This DNA region was cloned from Salmonella serotype Typhimurium and sequence analysis revealed the presence of a 6,105- bp open reading frame, designated shdA, whose product's deduced amino acid sequence displayed homology to that of AIDA-I from diarrheagenic Escherichia coli, MisL of serotype Typhimurium, and IcsA of Shigella flexneri. The shdA gene was located adjacent to xseA at 52 min, in a 30-kb DNA region which is not present in Escherichia coli K-12. A serotype Typhimurium shdA mutant was shed with the feces in reduced numbers and for a shorter period of time compared to its isogenic parent. A possible role for the shdA gene during the expansion in host range of S. enterica subspecies I to include warm-blooded vertebrates is discussed.

Original languageEnglish (US)
Pages (from-to)2720-2727
Number of pages8
JournalInfection and Immunity
Volume68
Issue number5
DOIs
StatePublished - May 2000
Externally publishedYes

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Salmonella enterica
Salmonella
Genes
Livestock
Poultry
DNA
Escherichia coli
Shigella flexneri
Amino Acid Sequence Homology
Reptiles
Host Specificity
Salmonella typhimurium
Feces
Open Reading Frames
Sequence Analysis
Vertebrates
Serogroup
Population

ASJC Scopus subject areas

  • Immunology

Cite this

The shdA gene is restricted to serotypes of Salmonella enterica subspecies I and contributes to efficient and prolonged fecal shedding. / Kingsley, Robert A.; Van Amsterdam, Karin; Kramer, Naomi; Baumler, Andreas J.

In: Infection and Immunity, Vol. 68, No. 5, 05.2000, p. 2720-2727.

Research output: Contribution to journalArticle

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