The Shaw-related potassium channel gene, Kv3.1, on human chromosome 11, encodes the type l K+ channel in T cells

S. Grissmer, S. Ghanshani, B. Dethlefs, John Douglas Mcpherson, J. J. Wasmuth, G. A. Gutman, M. D. Cahalan, K. G. Chandy

Research output: Contribution to journalArticle

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Abstract

T lymphocytes exhibit three distinct types of voltage-gated K+ channels, n, n', and l, that are distributed in the T cell lineage according to subset, as well as the cells' activation and developmental status. Type l K+ channels are found sparingly in cytotoxic T cells from normal mice and abundantly in a specific T cell subset (CD4- CD8- Thy1+) from mice with autoimmune disease. Here, we show that the mouse Kv3.1 gene, when expressed in Xenopus oocytes, encodes a channel with properties remarkably similar to those of the l-type channel. Kv3.1 transcripts were found in T cells isolated from the lymph nodes of MRL-lpr mice with systemic lupus erythematosus and in a human lymphoma cell line that also expresses the l channel phenotype. By these criteria, we conclude that Kv3.1 encodes the voltage-gated type l K+ channel in lymphocytes. The Kv3.1 gene maps to human chromosome 11; the related Kv1.1 and Kv3.2 genes are localized on human chromosome 12, while the IsK gene maps to human chromosome 21.

Original languageEnglish (US)
Pages (from-to)20971-20979
Number of pages9
JournalJournal of Biological Chemistry
Volume267
Issue number29
StatePublished - 1992

Fingerprint

Shaw Potassium Channels
Chromosomes, Human, Pair 11
T-cells
Potassium Channels
Human Chromosomes
Chromosomes
Genes
T-Lymphocytes
Voltage-Gated Potassium Channels
Inbred MRL lpr Mouse
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 21
T-Lymphocyte Subsets
Cell Lineage
Xenopus
Systemic Lupus Erythematosus
Lymphocytes
Autoimmune Diseases
Oocytes
Lymphoma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Grissmer, S., Ghanshani, S., Dethlefs, B., Mcpherson, J. D., Wasmuth, J. J., Gutman, G. A., ... Chandy, K. G. (1992). The Shaw-related potassium channel gene, Kv3.1, on human chromosome 11, encodes the type l K+ channel in T cells. Journal of Biological Chemistry, 267(29), 20971-20979.

The Shaw-related potassium channel gene, Kv3.1, on human chromosome 11, encodes the type l K+ channel in T cells. / Grissmer, S.; Ghanshani, S.; Dethlefs, B.; Mcpherson, John Douglas; Wasmuth, J. J.; Gutman, G. A.; Cahalan, M. D.; Chandy, K. G.

In: Journal of Biological Chemistry, Vol. 267, No. 29, 1992, p. 20971-20979.

Research output: Contribution to journalArticle

Grissmer, S, Ghanshani, S, Dethlefs, B, Mcpherson, JD, Wasmuth, JJ, Gutman, GA, Cahalan, MD & Chandy, KG 1992, 'The Shaw-related potassium channel gene, Kv3.1, on human chromosome 11, encodes the type l K+ channel in T cells', Journal of Biological Chemistry, vol. 267, no. 29, pp. 20971-20979.
Grissmer, S. ; Ghanshani, S. ; Dethlefs, B. ; Mcpherson, John Douglas ; Wasmuth, J. J. ; Gutman, G. A. ; Cahalan, M. D. ; Chandy, K. G. / The Shaw-related potassium channel gene, Kv3.1, on human chromosome 11, encodes the type l K+ channel in T cells. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 29. pp. 20971-20979.
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