The serologic response of horses to equine arteritis virus as determined by competitive enzyme-linked immunosorbent assays (c-ELISAs) to structural and non-structural viral proteins

H. M. Wagner, U. B R Balasuriya, Nigel J Maclachlan

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In an effort to further characterize the humoral immune response of horses to equine arteritis virus (EAV), direct and competitive enzyme-linked immunosorbent assays (c-ELISAs) were developed using monoclonal and polyclonal anti-sera to structural (GL, N and M) and non-structural (nsp1) viral proteins. A nsp1-specific monoclonal antibody was produced to facilitate development of a c-ELISA to this protein. Data obtained using the various c-ELISAs confirm that the M protein is a major target of the antibody response of horses to EAV. However, none of the c-ELISAs that were developed were as sensitive in detecting EAV-specific antibodies in horse sera as the existing serum neutralization test.

Original languageEnglish (US)
Pages (from-to)251-560
Number of pages310
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume26
Issue number4
DOIs
StatePublished - Jul 2003

Keywords

  • Anti-peptide serum
  • c-ELISA
  • Equine arteritis virus
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

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