The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum

Richard P Tucker, L. I. Binder, C. Viereck, B. A. Hemmings, A. I. Matus

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Mammalian microtubule-associated protein 2 (MAP2) exists in high-molecular-weight (M(r) ~ 280,000) and low-molecular-weight (M(r) ~ 70,000) forms, with the latter protein being more abundant in embryonic brain homogenates than in preparations from mature brain (Riederer and Matus, 1985). In the current study, we have shown that avian MAP2 also exists as both high- (M(r) ~ 260,000) and low-molecular-weight (M(r) ~ 65,000) forms whose relative abundance changes during brain maturation, indicating a conserved function for these proteins during vertebrate neuronal morphogenesis. Using indirect immunohistochemistry, we have determined the cellular distribution of the high- and low-molecular-weight forms of MAP2 in the developing avian cerebellum. In the embryonic cerebellum, low-molecular-weight MAP2 is found in the external granular layer and in epithelial cells. High-molecular-weight MAP2 is found only in neurons that have commenced dendrogenesis, i.e., Purkinje cells and neurons with in the internal granular layer. Thus, low-molecular-weight MAP2 is not only more abundant in embryonic nervous tissue than in the adult, but it also appears in glia and in differentiating neurons before the high-molecular-weight form. We have also shown that in the mature cerebellum high-molecular-weight MAP2 cannot be detected with monoclonal antibodies or polyclonal antisera in Purkinje cells dendrites. Polyclonal antisera against the regulatory subunit of the cAMP-dependent protein kinase, which is associated with MAP2 in the Purkinje cell dendrites of the rat, also fail to stain Purkinje cell dendrites in the mature quail cerebellum. This suggests that high-molecular-weight MAP2 may be necessary for the establishment of dendritis but is not necessary for the maintenance of dendritic form.

Original languageEnglish (US)
Pages (from-to)4503-4512
Number of pages10
JournalJournal of Neuroscience
Volume8
Issue number12
StatePublished - 1988
Externally publishedYes

Fingerprint

Microtubule-Associated Proteins
Cerebellum
Molecular Weight
Purkinje Cells
Dendrites
Immune Sera
Brain
Avian Proteins
Neurons
Nerve Tissue
Quail
Neocortex
Cyclic AMP-Dependent Protein Kinases
Morphogenesis
Neuroglia
Vertebrates
Proteins
Coloring Agents
Epithelial Cells
Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tucker, R. P., Binder, L. I., Viereck, C., Hemmings, B. A., & Matus, A. I. (1988). The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum. Journal of Neuroscience, 8(12), 4503-4512.

The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum. / Tucker, Richard P; Binder, L. I.; Viereck, C.; Hemmings, B. A.; Matus, A. I.

In: Journal of Neuroscience, Vol. 8, No. 12, 1988, p. 4503-4512.

Research output: Contribution to journalArticle

Tucker, RP, Binder, LI, Viereck, C, Hemmings, BA & Matus, AI 1988, 'The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum', Journal of Neuroscience, vol. 8, no. 12, pp. 4503-4512.
Tucker, Richard P ; Binder, L. I. ; Viereck, C. ; Hemmings, B. A. ; Matus, A. I. / The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum. In: Journal of Neuroscience. 1988 ; Vol. 8, No. 12. pp. 4503-4512.
@article{78da7a6881f341f497108f0956efe4ea,
title = "The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum",
abstract = "Mammalian microtubule-associated protein 2 (MAP2) exists in high-molecular-weight (M(r) ~ 280,000) and low-molecular-weight (M(r) ~ 70,000) forms, with the latter protein being more abundant in embryonic brain homogenates than in preparations from mature brain (Riederer and Matus, 1985). In the current study, we have shown that avian MAP2 also exists as both high- (M(r) ~ 260,000) and low-molecular-weight (M(r) ~ 65,000) forms whose relative abundance changes during brain maturation, indicating a conserved function for these proteins during vertebrate neuronal morphogenesis. Using indirect immunohistochemistry, we have determined the cellular distribution of the high- and low-molecular-weight forms of MAP2 in the developing avian cerebellum. In the embryonic cerebellum, low-molecular-weight MAP2 is found in the external granular layer and in epithelial cells. High-molecular-weight MAP2 is found only in neurons that have commenced dendrogenesis, i.e., Purkinje cells and neurons with in the internal granular layer. Thus, low-molecular-weight MAP2 is not only more abundant in embryonic nervous tissue than in the adult, but it also appears in glia and in differentiating neurons before the high-molecular-weight form. We have also shown that in the mature cerebellum high-molecular-weight MAP2 cannot be detected with monoclonal antibodies or polyclonal antisera in Purkinje cells dendrites. Polyclonal antisera against the regulatory subunit of the cAMP-dependent protein kinase, which is associated with MAP2 in the Purkinje cell dendrites of the rat, also fail to stain Purkinje cell dendrites in the mature quail cerebellum. This suggests that high-molecular-weight MAP2 may be necessary for the establishment of dendritis but is not necessary for the maintenance of dendritic form.",
author = "Tucker, {Richard P} and Binder, {L. I.} and C. Viereck and Hemmings, {B. A.} and Matus, {A. I.}",
year = "1988",
language = "English (US)",
volume = "8",
pages = "4503--4512",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "12",

}

TY - JOUR

T1 - The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum

AU - Tucker, Richard P

AU - Binder, L. I.

AU - Viereck, C.

AU - Hemmings, B. A.

AU - Matus, A. I.

PY - 1988

Y1 - 1988

N2 - Mammalian microtubule-associated protein 2 (MAP2) exists in high-molecular-weight (M(r) ~ 280,000) and low-molecular-weight (M(r) ~ 70,000) forms, with the latter protein being more abundant in embryonic brain homogenates than in preparations from mature brain (Riederer and Matus, 1985). In the current study, we have shown that avian MAP2 also exists as both high- (M(r) ~ 260,000) and low-molecular-weight (M(r) ~ 65,000) forms whose relative abundance changes during brain maturation, indicating a conserved function for these proteins during vertebrate neuronal morphogenesis. Using indirect immunohistochemistry, we have determined the cellular distribution of the high- and low-molecular-weight forms of MAP2 in the developing avian cerebellum. In the embryonic cerebellum, low-molecular-weight MAP2 is found in the external granular layer and in epithelial cells. High-molecular-weight MAP2 is found only in neurons that have commenced dendrogenesis, i.e., Purkinje cells and neurons with in the internal granular layer. Thus, low-molecular-weight MAP2 is not only more abundant in embryonic nervous tissue than in the adult, but it also appears in glia and in differentiating neurons before the high-molecular-weight form. We have also shown that in the mature cerebellum high-molecular-weight MAP2 cannot be detected with monoclonal antibodies or polyclonal antisera in Purkinje cells dendrites. Polyclonal antisera against the regulatory subunit of the cAMP-dependent protein kinase, which is associated with MAP2 in the Purkinje cell dendrites of the rat, also fail to stain Purkinje cell dendrites in the mature quail cerebellum. This suggests that high-molecular-weight MAP2 may be necessary for the establishment of dendritis but is not necessary for the maintenance of dendritic form.

AB - Mammalian microtubule-associated protein 2 (MAP2) exists in high-molecular-weight (M(r) ~ 280,000) and low-molecular-weight (M(r) ~ 70,000) forms, with the latter protein being more abundant in embryonic brain homogenates than in preparations from mature brain (Riederer and Matus, 1985). In the current study, we have shown that avian MAP2 also exists as both high- (M(r) ~ 260,000) and low-molecular-weight (M(r) ~ 65,000) forms whose relative abundance changes during brain maturation, indicating a conserved function for these proteins during vertebrate neuronal morphogenesis. Using indirect immunohistochemistry, we have determined the cellular distribution of the high- and low-molecular-weight forms of MAP2 in the developing avian cerebellum. In the embryonic cerebellum, low-molecular-weight MAP2 is found in the external granular layer and in epithelial cells. High-molecular-weight MAP2 is found only in neurons that have commenced dendrogenesis, i.e., Purkinje cells and neurons with in the internal granular layer. Thus, low-molecular-weight MAP2 is not only more abundant in embryonic nervous tissue than in the adult, but it also appears in glia and in differentiating neurons before the high-molecular-weight form. We have also shown that in the mature cerebellum high-molecular-weight MAP2 cannot be detected with monoclonal antibodies or polyclonal antisera in Purkinje cells dendrites. Polyclonal antisera against the regulatory subunit of the cAMP-dependent protein kinase, which is associated with MAP2 in the Purkinje cell dendrites of the rat, also fail to stain Purkinje cell dendrites in the mature quail cerebellum. This suggests that high-molecular-weight MAP2 may be necessary for the establishment of dendritis but is not necessary for the maintenance of dendritic form.

UR - http://www.scopus.com/inward/record.url?scp=0024205197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024205197&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 4503

EP - 4512

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 12

ER -