The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa

Hossein Gouran, Hyrum Gillespie, Rafael Nascimento, Sandeep Chakraborty, Paulo A. Zaini, Aaron Jacobson, Brett S. Phinney, David Dolan, Blythe P. Durbin-Johnson, Elena S. Antonova, Steven E. Lindow, Matthew S. Mellema, Luiz R. Goulart, Abhaya M. Dandekar

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Pierce's disease (PD) is a deadly disease of grapevines caused by the Gram-negative bacterium Xylella fastidiosa. Though disease symptoms were formerly attributed to bacteria blocking the plant xylem, this hypothesis is at best overly simplistic. Recently, we used a proteomic approach to characterize the secretome of X. fastidiosa, both in vitro and in planta, and identified LesA as one of the pathogenicity factors of X. fastidiosa in grapevines that leads to leaf scorching and chlorosis. Herein, we characterize another such factor encoded by PD0956, designated as an antivirulence secreted protease "PrtA" that displays a central role in controlling in vitro cell proliferation, length, motility, biofilm formation, and in planta virulence. The mutant in X. fastidiosa exhibited reduced cell length, hypermotility (and subsequent lack of biofilm formation) and hypervirulence in grapevines. These findings are supported by transcriptomic and proteomic analyses with corresponding plant infection data. Of particular interest, is the hypervirulent response in grapevines observed when X. fastidiosa is disrupted for production of PrtA, and that PD-model tobacco plants transformed to express PrtA exhibited decreased symptoms after infection by X. fastidiosa.

Original languageEnglish (US)
Article number31098
JournalScientific Reports
Volume6
DOIs
StatePublished - Aug 5 2016

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Xylella fastidiosa
biofilm
cell growth
proteinases
pathogenicity
Pierce's disease
Plantae
proteomics
signs and symptoms (plants)
disease models
chlorosis
transcriptomics
Gram-negative bacteria
infection
xylem
cell proliferation
tobacco
virulence
mutants
bacteria

ASJC Scopus subject areas

  • General

Cite this

Gouran, H., Gillespie, H., Nascimento, R., Chakraborty, S., Zaini, P. A., Jacobson, A., ... Dandekar, A. M. (2016). The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa. Scientific Reports, 6, [31098]. https://doi.org/10.1038/srep31098

The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa. / Gouran, Hossein; Gillespie, Hyrum; Nascimento, Rafael; Chakraborty, Sandeep; Zaini, Paulo A.; Jacobson, Aaron; Phinney, Brett S.; Dolan, David; Durbin-Johnson, Blythe P.; Antonova, Elena S.; Lindow, Steven E.; Mellema, Matthew S.; Goulart, Luiz R.; Dandekar, Abhaya M.

In: Scientific Reports, Vol. 6, 31098, 05.08.2016.

Research output: Contribution to journalArticle

Gouran, H, Gillespie, H, Nascimento, R, Chakraborty, S, Zaini, PA, Jacobson, A, Phinney, BS, Dolan, D, Durbin-Johnson, BP, Antonova, ES, Lindow, SE, Mellema, MS, Goulart, LR & Dandekar, AM 2016, 'The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa', Scientific Reports, vol. 6, 31098. https://doi.org/10.1038/srep31098
Gouran H, Gillespie H, Nascimento R, Chakraborty S, Zaini PA, Jacobson A et al. The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa. Scientific Reports. 2016 Aug 5;6. 31098. https://doi.org/10.1038/srep31098
Gouran, Hossein ; Gillespie, Hyrum ; Nascimento, Rafael ; Chakraborty, Sandeep ; Zaini, Paulo A. ; Jacobson, Aaron ; Phinney, Brett S. ; Dolan, David ; Durbin-Johnson, Blythe P. ; Antonova, Elena S. ; Lindow, Steven E. ; Mellema, Matthew S. ; Goulart, Luiz R. ; Dandekar, Abhaya M. / The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa. In: Scientific Reports. 2016 ; Vol. 6.
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