Abstract
We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD+ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 470-478 |
| Number of pages | 9 |
| Journal | MGG Molecular & General Genetics |
| Volume | 232 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 1992 |
| Externally published | Yes |
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Keywords
- P22 anti-RecBC functions
- recBCD
- recJ
- UV sensitivity
ASJC Scopus subject areas
- Genetics
Cite this
The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD+ hosts. / Mahan, Michael J.; Casadesus, Josep; Roth, John R.
In: MGG Molecular & General Genetics, Vol. 232, No. 3, 04.1992, p. 470-478.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD+ hosts
AU - Mahan, Michael J.
AU - Casadesus, Josep
AU - Roth, John R.
PY - 1992/4
Y1 - 1992/4
N2 - We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD+ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.
AB - We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD+ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.
KW - P22 anti-RecBC functions
KW - recBCD
KW - recJ
KW - UV sensitivity
UR - http://www.scopus.com/inward/record.url?scp=0026524575&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026524575&partnerID=8YFLogxK
U2 - 10.1007/BF00266252
DO - 10.1007/BF00266252
M3 - Article
C2 - 1588916
AN - SCOPUS:0026524575
VL - 232
SP - 470
EP - 478
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 3
ER -