The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD+ hosts

Michael J. Mahan; Josep Casadesus; John R. Roth

doi:10.1007/BF00266252

The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD⁺ hosts

Michael J. Mahan, Josep Casadesus, John R. Roth

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD⁺ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.

Original language	English (US)
Pages (from-to)	470-478
Number of pages	9
Journal	MGG Molecular & General Genetics
Volume	232
Issue number	3
DOIs	https://doi.org/10.1007/BF00266252
State	Published - Apr 1992
Externally published	Yes

Keywords

P22 anti-RecBC functions
recBCD
recJ
UV sensitivity

ASJC Scopus subject areas

Genetics

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10.1007/BF00266252

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Cite this

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title = "The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD+ hosts",

abstract = "We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD+ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.",

keywords = "P22 anti-RecBC functions, recBCD, recJ, UV sensitivity",

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year = "1992",

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T1 - The Salmonella typbimurium RecJ function permits growth of P22 abc phage on recBCD+ hosts

AU - Mahan, Michael J.

AU - Casadesus, Josep

AU - Roth, John R.

PY - 1992/4

Y1 - 1992/4

N2 - We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD+ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.

AB - We describe recJ mutants of Salmonella typhimurium. The recJ gene maps between sufD and serA (min 62) and is transcribed counterclockwise. Unlike recJ mutants of Escherichia coli, recJ strains of S. typhimurium are sensitive to irradiation with UV light. This sensitivity is equivalent to or greater that that displayed by recBCD mutant strains. The residual ability of phage P22 abc (anti-recBCD) mutants to form plaques on recBCD+ strains is eliminated in recJ hosts. Thus host RecJ function appears to substitute for the anti-RecBCD functions of phage P22 and may serve to limit RecBCD activity.

KW - P22 anti-RecBC functions

KW - recBCD

KW - recJ

KW - UV sensitivity

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